Match the terms associated with tne polymerase chain reaction with their correct descriptions.

RNA-dependent RNA polymerase performs which of the following functions? O 1) Uncoats the viral genome 2) transcribes retroviral RNA genomes into DNA 3) Replicates RNA into RNA O 4) Replicates DNA into RNA 5) Shuttles RNA genomes into the nucleus for assembly

In the Hershey Chase experiment, why was the localization of radio-labeled phage of macromolecules in the cytoplasm the reason that the genetic material identity was proved.

Briefly describe the lytic and lysogenic cycles of bacteriophages.In your answer, explain what a prophage is?

With respect to DNA and RNA polymerases which statement is correct? A) Only DNA polymerases are DNA template driven. B) Only DNA polymerases require primers. OC) Only RNA polymerases are DNA template driven. OD) Only RNA polymerases require primers. E) Both require a DNA template, activated monomer units and a primer

The {choose } options are transformation, conjugation, or transduction.

Can you please answer 18, and 19

O O O A new virus is discovered. Its RNA genome encodes several proteins, including a reverse transcriptase enzyme. What can you conclude about replication of this virus? Its replication involves a double-stranded RNA intermediate Its replication involves a DNA copy of its genome Its replication depends entirely on cellular enzymes Its replication is entirely independent of cellular enzymes 00 A deficiency in Base Excision Repair (BER) would primarily sensitize you to mutations caused by radiation, X-rays O Chemicals in smoke, and UV radiation O Unavoidable, spontaneously occurring DNA damage

Consider a hypothetical phage whose DNA replicates exclusively by rolling circle replication. A phage with radioactive DNA in both strands infects a bacterium and is allowed to replicate in a nonradioactive medium. Assume that only daughter DNA from the elongated branch ever gets packaged into progeny particles.    What fraction of the parental radioactivity will appear in the progeny phage? How many progeny phage will contain radioactive DNA? What is the fundamental difference between the initiation of θ replication and that of the rolling circle?

Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell.   Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infection