How much energy is required to pump a mole of protons in a mitochondria with the following conditions? pH of matrix = 8.2, pH of intermembrane space = 7.4, membrane potential of - 65 mv (N-side relative to P-side), temperature 37C. Express answer to the nearest 0.1 kJ/mole.

Mutations in adenylate kinase have led to a hyperactive enzyme that ultimately ends up elevating ADP levels in a cell.Calculate the EC (energy charge) given the following atypical adenylate concentrations for the cell containing the mutant adenylate kinase: ATP = 0.5 ??��, ADP = 12.2 ??��, AMP = 80 ??��.

Human lysozyme is an enzyme active in lysosomes.  At approximately what pH would you expect this enzyme to display maximal activity?   Question options:   enzyme activity should be reasonably similar at all of these pH values   9   7   5   11

The transport system of lactose in E.coli is shown below: Lactose transporter H+ H+ Lactose (outside) H+ H+ H+ H+ Proton pump H* + ++ Lactose (inside) ADP + P, H* H+ ATP H* A) For each of the following statements determine if they are true (T) or false (F). • The primary active transport of H" via proton pump, establishes a proton gradient across the membrane. 1 Select ) • The intracellular pH is lower than the extracellular pH. [Select] - Lactose transporter is an example of antiport. Select] Transport of lactose against its concentration gradient is entirely dependent on inflow of H" down their electrochemical gradient. ISelect] B) What would happen with the transport of Lactose if an antimicrobial agent triclosan is added? Triclosan is a proton onophore. I Select]