How can molecular structural analysis be accomplished with electrospray ionization mass spectrometry?

Q: Discuss the principle behind Salkowski test, reaction with ammonium ferrothiocyanate, and…

A: Introduction: Lipids are defined as esters of alcohol and fatty acids which are water-insoluble and…

Q: How can a mass spectrometer be used to identify the protein/s excised from a 2D gel?

A: Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio…

Q: What is the significance of UV unwrapping in the texturing process of 3D models?

A: It plays a pivotal role in the process of texturing 3D models. It includes applications in the field…

Q: Describe the Lidcombe program.

A: Lidcombe program was developed at Sydney University. This program was introduced for the treatment…

Q: How do you verify amino acid sequences with proteins and species with BLAST?

A: Bioinformatics is an interdisciplinary field of study which has diverse application in the…

Q: List all the components and explain their purpose in the reaction mixture used for the dideoxy DNA…

A: The method of evaluating the sequence of nucleotides (As, Ts, Cs, and Gs) in a portion of DNA is DNA…

Q: What is known as electropherogram ?

A: An analytical procedure that is used for the separation of fragments of DNA or RNA, in terms of…

Q: Compare and contrast the following protein characterization techniques in terms of the principles…

A: Precipitation happens when the pH or hydrophobicity of a protein affects interactions with the…

Q: n SDS PAGE the resolving gel :   is 4%(w/v) acrylamide,PH )6.8   is 6-20% (w/v) acrylamide…

A: SDS PAGE stands for Sodium Dodecyl Sulphate Poly-Acrylamide Gel Electrophoresis. It is a technique…

Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?

A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…

Q: Outline the method of in situ hybridization.

A: In molecular biology, hybridization is defined as a technique in which RNA or single-stranded DNA…

Q: Describe the two general types of protein microarrays. What aretheir possible applications?

A: A protein microarray is also known as the protein chip, is the high throughput method that is used…

Q: How do microarrays work?

A: A microarray is a laboratory tool used to detect the expression of thousands of genes at the same…

Q: What is oligonucleotide microarray?

A: An array is an orderly arrangement of samples where matching of known and unknown DNA samples is…

Q: Look at what sections are present. Which sections does this paper have such as citation, abstract,…

A: Refer to the solution

Q: I understand that microarrays are being used to define the molecular abnormality and the prognosis…

A: Leukemia and types

Q: How are molecular probes prepared?

A: Molecular probes are small segments of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), which…

Q: How do you determine protein concentration using bicinchoninic acid assay?

A: Proteins are polymers that are composed of amino acids. Amino acids are in turn made up of a central…

Q: 2.) An unknown amino acid was subjected to paper chromatography with five amino acid standards in…

A: For Thin layer chromatography, we define Retension factor (Rf) as ratio of distance travelled by…

Q: Immunofluorescence and Fluorescent live cell imaging techniques can both be used to determine…

A: Immunofluorescence is a technique in which fluorochrome labeled-antibodies are used to detect the…

Q: What are the limitations of doing a molecular identification of bacteria?

A: Bacteria are the small single-celled organisms. They are found almost everywhere on Earth and are…

Q: How can gel-filtration chromatography be used to arrive at an estimate of the molecular weight of a…

A: Chromatography is an important analytical technique that contributes to the separation,…

Q: What makes macromolecules move through the gel in electrophoresis? 2. What determines the speed at…

A: The physical bodies of life forms are composed of 4 main macromolecules. They are proteins, nucleic…

Q: Explain the extraction method and the procedure for extraction of Echinolone by using…

A: Chromatography works on the principle of molecules in a mixture being applied to a surface or a…

Q: What is mass fragmentography? How is this performed?

A: Mass fragmentography is also known by the name mass fragmentometric analysis or mass fragmentography…

Q: Make an analysis of the electrophoresis data below. Comment on the purity, number of subunits, and…

A: Native gel electrophoresis is the process of separation without adding any denaturing agent like…

Q: What type of probes must be used for super-resolutionmicroscopy and why?

A: The super-resolution microscopy involves a series of techniques that allow high-resolution images.…

Q: What are the benefits and disadvantages of IMAC for purifying proteins?

A: The purification of the proteins is performed by the use of different chromatographic procedures…

Q: The SDS-PAGE gel matrix used to separate proteins by electrophoresis is composed of: A)…

A: Biological molecules are separated using the gel electrophoresis which is based on size and other…

Q: Which of the following are true regarding assay attributes? List all that are true. a) Accuracy…

A: Assays are widely used in biological and medical research to measure the concentration of biological…

Q: What are the principles of Chelex extraction and solid-phase DNA extraction methods?

A: To explain the principles of Chelex extraction and solid-phase DNA extraction methods The principle…

Q: List four reasons for the development of pulsed field gel electrophoresis

A: Electrophoresis is a general term that describes the migration and separation of charged particles…

Q: What is DNA fingerprinting? Define and differentiate VNTR and Probe and what is the applications of…

A: Step 1 DNA or deoxyribonucleic acid occurs in living systems and can function as genetic materials.…

Q: What is the difference between a random sample and a composite sample? When would each be used?

A: Sampling is the process of obtaining a small representative of a large population which can be…

Q: A mixture of amino acids that do not separate sufficiently when a single technique is used can often…

A: Chromatography is the method of separating a mixture of components into individual components by…

Q: Name some techniques/approaches (name, describe the major steps, how to analyze/interpret results)…

A: Proteins: Proteins are polymers that contain hundreds or even thousands of amino acids that are…

Q: Describe step by step process of: a. Ion-exchange Chromatography b. Hydrophobic Interaction…

A: Introduction: Chromatography is a physical method for the separation of compounds. Tswet, a Russian…