Q: Why is it not safe to assume that two microorganisms with the same G + C content belong to the same…
A: The GC content helps in establishing the relatedness between species when there is a similarity in…
Q: enrichment culture to isolate aerobic and anaerobic nitrogen-fixing bacteria, sulfate-reducing…
A: Enrichment culture: This type of culture containing certain growth media ,allows and favours the…
Q: What advantages do solid media offer for the isolation ofmicroorganisms?
A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…
Q: hat is the advantage of culture-independent analysis over culture-dependent analysis of…
A: An unknown microorganism can be identified and classified in a variety of ways. They are divided…
Q: What is a micrometer (μm )? Is it really possible to see microbes with this dimension using…
A: Microbes are often tiny, even when compared to minuscule mammal cells. To observe them, the…
Q: You culture an unknown bacterial species on MSA media. The organism grows well, and the media turns…
A: MSA is a type of medium that is selective as well as differential and is extensively used in…
Q: Is the Tryptoc Soy Agar (TSA) a complex defined medium? Explaining based on its composition? Is TSA…
A: Trypticase soy agar (TSA) and Trypticase soy broth is a growth media for the culturing of bacteria.
Q: Is it acceptable to give a formal name to a microbe that hasn't been isolated and cultivated? What…
A: There are two main microbiology methods used for isolating bacteria from a clinical sample in order…
Q: Explain the principle involved in O.D determination of microbial populations using the…
A: A spectrophotometer is an instrument that can determine the concentration of a compound or solution…
Q: What are the physical methods of microbial control
A: Control of microorganisms is fundamental to forestall the transmission of illnesses and disease,…
Q: How to culture the media of L. bulgaricus and S. thermophiles properly by using aseptic technique?
A: For the culture of L. bulgaricus and S. thermophiles RCA (Reinforced clostridial) agar and ST…
Q: Describe fully how to culture the media of L. bulgaricus and S. thermophiles properly by using…
A: The thermophilic lactic acid bacteria (LAB) Lactobacillus bulgaricus and Streptococcus thermophilus…
Q: What are the different techniques use to observe the morphology and motility of microorganisms
A: Light microscope is used for determining motility of microorganism or some special morphological…
Q: Why are semi-solid media such as TTC deeps suitable to test motility of bacteria?
A: There are several parameters which need to be studied in microbiology about different…
Q: why it is useful to plot the microbial growth from a growing microbial culture?
A: The term microbial growth refers to the growth of a population of microbe, it is an increase in the…
Q: How does an aerotolerant anaerobe differ from a microaerophile?
A: Microorganisms are classified into different types based on the requirement of oxygen and are…
Q: How to produce high yield of biomass of facultative anaerobic bacterium in laboratories?
A: Facultative anaerobic organism It refers to the organism that generates ATP through the process of…
Q: How do facultative anaerobes differ from obligate anaerobes? How do they differ from aerobes?
A: Anaerobes and aerobes are the different types of organisms present in the environment. They can be…
Q: What is the hurdle technology used to destry the microbial load in squash soup?
A: Squash soup is a liquid dish and it is present with High nutrients and low calorie i.e low carbs.…
Q: What is the purpose of the quadrant streak plate method?
A:
Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…
A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…
Q: How to prepare wet mounts and hanging drop mounts of live specimen in microbial motility?
A: Wet mounts and hanging drop mounts of live specimen are used in microbial motility.
Q: Name five functional traits of microorganisms. What dose the functional diversity describe? Do we…
A: Functional traits of the microbes describe individually expressed phenotypes or characteristics of…
Q: hich method for introducing microbes on media uses an inoculation loop and the bacterial sample is…
A: Answer. The four-quadrant streak method is used for introducing microbes on media using an…
Q: If you had a mixed culture that contained a Gram-negative enteric bacterium and Gram-positive…
A: MSA or mannitol sale agar used in microbiology. It encourages growth of some group of bacteria…
Q: What makes agar good support for microbial growth?
A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…
Q: Why is Escherichia coli used as an indicator organism inmicrobial analyses of water?
A: The well-known gram-negative bacteria are E. coli (Escherichia coli).
Q: Discuss briefly the phases of Microbial Growth Curve and why it is useful to plot the microbial…
A: For microbial growth many factors are important such as nutrients, temperature, pH, moisture and…
Q: For what the stirred tank bioreactors have been designed?
A: Bioreactors are generally used for microbial fermentation processes. It has a headspace volume and…
Q: How does the differences in the physical and chemical structure of Gram-positive and Gram-negative…
A: The bacterial cells are categorized into gram-positive and gram-negative mainly depending on their…
Q: determination of microbial motility, why do you think the microorganisms are ubiquitous?
A: Solution : The motility test is used to determine whether an organism is motile or non-motile.…
Q: How does an autoclave sterilize instruments? What is used to dry glassware and at the same time
A: Autoclaving is the best method for sterilizing glass instruments. Sterilizing means removal of all…
Q: In determination of microbial motility, what is the value of a hanging-drop perparation? what is the…
A: Hanging drop is a motility testing which helps microbiologist to view motility of bacterium.
Q: Why is it Koch's postulates cannot be used for the microbe E. Coli. What limitations does E.coli…
A: The virulence and pathogenicity is considered as the ability of bacteria to cause disease. Robert…
Q: Which of the following groups of bacteria require or will use oxygen for respiration i. obligate…
A: Bacteria are defined as unicellular prokaryotes. They can survive or live in extreme environmental…
Q: Discuss how can some anaerobes be maintained in pure cultures?
A: Introduction :- A pure (or axenic) culture is a population of cells or multicellular creatures that…
Q: Describe what three strategies are used to identify microorganisms?
A: Microorganisms: Microorganisms are tiny organisms that are not visible through an unaided eye.…
Q: Which techniques may be used for viable counting methods of microbial measurements? Explain.
A: To identify the microorganism, it must first be placed into a medium to commence development, and…
Q: Describe methods used to grow microbes anaerobically and under high CO2 conditions
A: An anaerobic organism does not require oxygen for its growth. It may react negatively or may even…
Q: A bacterial species X is inoculated in a screw capped test tube containing the broth media and…
A: The aerotolerant Species are those which can actually survive without oxygen. These are however, not…
Q: What is G+C content of a bacteria? How it can be determined? Why it is not safe to assume that…
A: GC-content is the percentage of nitrogenous bases in DNA or RNA molecule that are either guanine or…
Q: ar contains salt to prevent the growth of most microbes and mannitol plus a pH indicator to detect…
A: In order to produce and culture different types of microbial species, a culture media is required.…
Q: What are the advantages and disadvantages of plate count technique over other methods of quantifying…
A: Plate count is a technique in which bacteria are counted by growing on the agar plates. Total…
Q: What is an extremophile? What are some of the categories of extremophile microbes?
A: All organisms are categorized into three domains. They are archaea, eukaryote, and bacteria. The…
Q: Discuss the THREE (3) main stages crucial to microbial leaching of copper from a low-grade ore,…
A: Bioleaching involes microorganisms .Main copper minerals include sulfides, (CuFeS2) chalcopyrite,…
Discuss, how is it possible to isolate both aerobes and anaerobes using streak plates?
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- What advantages do solid media offer for the isolation of microorganisms? How will you check the purity of an isolate?https://courses.lumenlearning.com/microbiology/chapter/controlling-microbial-growth/ Read this Article about Controlling Microbial Growth and answer the following questions: Which is most effective at removing microbes from a product: sanitization, degerming, or sterilization? Explain. What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name at least two factors that can compromise the effectiveness of a disinfecting agent.What advantages do solid media offer for the isolation ofmicroorganisms?
- Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?What is the advantage of using Molecular data compared to plated culture or biochemical techniques in identifying bacterial species, especially for oral microbes ?Discuss fresh, living preparations vs fixed preparations. What are the advantages and disadvantages of each technique in a microbiology laboratory?
- Can simple technique be used to identify more than the morphological characteristics of microorganisms? Explain.Describe how specimen collection is performed using aseptic techniques to isolate medically significant bacteria and identify these clinically important bacterial species, Escherichia coli, Streptococcus pneumonia, Haemophilus influenzae and Streptococcus pyogenes by using different staining techniques and biochemical tests. Evaluate the effectiveness of different biochemical techniques used to identify different bacterial species.What are advantages and disadvantages of solid phase bioremediation technique?
- why it is useful to plot the microbial growth from a growing microbial culture?Based on the knowledge acquired about the conservation and propagation of microorganisms, propose methodologies for:a) conservation of stock culture (used infrequently) of baker's yeast (S. cerevisiae);b) conservation of the working culture (used daily) of the baker's yeast;c) activation (1st stage of propagation) of the work culture;d) propagation of cultures until inoculating a 100 L reactor toi) production of baker's yeast;ii) ethanol production.). Consider a culture medium on which only gram-positive organisms such as Staphylococcus aureus colonies can grow due to an elevated NaCl level. A yellow halo surrounds the growth, indicating the bacterium fermented sugar in the medium, decreasing the pH as a result and changing the color of a pH indicator chemical. This type of medium would be referred to as a(n): A) Selective medium. B) Differential medium. C) Enrichment culture. D) Selective and differential medium. E) Differential and enrichment culture.