How does recombination in bacteria differ from recombination in eukaryotes?

E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interference

Which of the following replication enzymes will most likely be involved in COVID19 RNA replication?  a) Helicase, Polymerase, Ligase  b) SSB protein, Polymerase, Ligase c) Topoisomerase, Primase, Ligase  d) Helicase, SSB protein, Primase  e) Topoisomerase, Polymerase, Primase

choose one answer

The percentage of the agarose gel is important because a) it influences the rate of migration of the fragments b) it may cause some DNA molecules to replicate c) some DNA nucleotides may degraded due to chemical reactions with the gel  d) some DNA molecules may sink to the bottom of the well and not migrate What is the purpose of a buffer used in electrophoresis? a) Increase/decrease the current b) Conduct the current and protect the samples c) Disrupt the bands d) Stop the band migration Which of the following is an additional use of the gel electrophoresis technique? a) To express DNA b) To separate RNA c) To amplify RNA d) To amplify genes

DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a– b– c– d– e–. Pairs of genes are checked for co-transformation and results shown below are obtained. Based on these results, what is the order of the genes on the bacterial chromosome? please show how you worked out the order by diagraming, thank you! Pair of genes     Co-transformation a+ and b+         no a+ and c+         no a+ and d+        yes a+ and e+        yes b+ and c+        yes b+ and d+         no b+ and e+         no     c+ and d+        no c+ and e+        yes d+ and e+        no

15. Which of the following statement is Not true?  a) Plasmids are circular DNA molecules b) Plasmids are able to replicate independently of the chromosome c) Plasmids are much smaller than the bacterial chromosome d) Plasmids are used in genetic engineering techniques e) Plasmids are liner, double stranded molecules

Define nucleoid and plasmids

A reciprocal translocation occurs in an individual between chromosomes 4 and 18, and this translocation causes a disease in the individual. What is the most likely reason chromosome mutation causes a disease? a) The translocation causes a break in the chromosome in a gene regulatory region. b) Chromosomes with a translocation cannot replicate. c) An individual with a translocation has some genes duplicated. d) An individual with a translocation has some genes missing.

In conjugation, F+ cells O 1) can transfer DNA only to other F+ cells. O 2) do not have conjugation pili. O3) contain "jumping genes." O 4) serve as recipient cells. O5) O 5) contain DNA R factors that can be shared.