10. The baker's yeast Saccharomyces cerevisiae is able to make uracil from simpler compounds (biosynthesis). A scientist doing research on pyrimidine biosynthesis isolated four mutant strains of yeast that were unable to make their own uracil. These strains are called ura- mutants. To determine if the different strain had mutations in the same gene, or in different genes, she performed complementation tests. She mated all of the strains in all of the possible combinations, and then determined if the hybrids had a ura- phenotype, or if they were normal wild type. The results are given in the table below. A "-" means that when the two strains were mated, their hybrid was still ura-. A "+" means that when the two strain were mated, their hybrid was normal wild type and able to make its own uracil. strain 1 strain 2 strain 3 strain 4 strain 1 strain 2 + strain 3 + strain 4 + + + A. How many different genes are represented by these mutant strains? B. Which mutant strains have mutations in the same gene, and which strains have mutations in genes that are different from all of the others?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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How did you figure out strain 1 has a mutation in the same gene?