Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Concept explainers

Microbial Isolation
Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.
Question

Given:

Enterobacteriaceae, Vibrionaceae, Pasteurellaceae and other organisms such as Calymmatobacterium, Cardiobacterium, Chromobacterium, Eikenella, Gardnerella, Streptobacillusand Zymomonas

 

Test

Unknown Isolate B

1

Luminescence

Positive

2

Cell Shape

Straight Rods

3

Gram Staining

Negative

4

Oxygen Requirement

Facultative Anaerobe

5

Motility          (Hanging

Drop)

Motile

6

Growth at

0% NaCl

Negative

7

3% NaCl

Positive

  8             

6 % NaCl                                     

Positive                

Attached is the sample

B. General Identification Scheme Unknown 5 Gram Stain Gram Negative Gram Positive Bacillus subtilis Staphylocoçcus oureus Escherichia coli Proteus vulgaris Pseudomonas oeruginosa Chromobacterium Methyl-Red test Yellow (Negative) Red (Positive) Stophylococcus dureus Citrate Utlization Test Green (Negative) Staphylocpccus aureus Blue Positive) Catalase Production No bubble (Negative) BUBBLE Tormation (Positive) Staphylocoçcus aureus Coagulase Test No Cloflormation Clot formation (Positive) Stophylo Starch Hydrolysis (Negative) No Clear Zone (negative) Staphylococcus quce Gelatin Hydrolysis Clear Zone (positive) Solid Negative) Liquid (Positive) Stopnylopoctus guTEUS Casein Hydrolysis Opaque (Negative) Zone of proteolysis (positive Staphylococcus eureus
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Transcribed Image Text:B. General Identification Scheme Unknown 5 Gram Stain Gram Negative Gram Positive Bacillus subtilis Staphylocoçcus oureus Escherichia coli Proteus vulgaris Pseudomonas oeruginosa Chromobacterium Methyl-Red test Yellow (Negative) Red (Positive) Stophylococcus dureus Citrate Utlization Test Green (Negative) Staphylocpccus aureus Blue Positive) Catalase Production No bubble (Negative) BUBBLE Tormation (Positive) Staphylocoçcus aureus Coagulase Test No Cloflormation Clot formation (Positive) Stophylo Starch Hydrolysis (Negative) No Clear Zone (negative) Staphylococcus quce Gelatin Hydrolysis Clear Zone (positive) Solid Negative) Liquid (Positive) Stopnylopoctus guTEUS Casein Hydrolysis Opaque (Negative) Zone of proteolysis (positive Staphylococcus eureus
Test Unknown Isolate A 1 Cell Shape Short Straight Rods, not in chain Gram Staining Spore Formation Oxygen Requirement Pigmentation Growth in ordinary media at 37°C Negative Non-Spore Forming Facultative anaerobe 3 4 Non-chromogenic 6 Positive 7 Flagella Peritrichous 8 Nitrate Reduction Positive Oxidase Negative Negative 9 Gelatinase Methyl Red VogesProskauer 10 Positive 11 Negative Negative Negative 12 Citrate 13 Pectinase 14 Indole Positive Phenylalanine deaminase Utilization of 15 Negative Positive (fermentative) Positive (fermentative) 16 Glucose 17 Lactose
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Transcribed Image Text:Test Unknown Isolate A 1 Cell Shape Short Straight Rods, not in chain Gram Staining Spore Formation Oxygen Requirement Pigmentation Growth in ordinary media at 37°C Negative Non-Spore Forming Facultative anaerobe 3 4 Non-chromogenic 6 Positive 7 Flagella Peritrichous 8 Nitrate Reduction Positive Oxidase Negative Negative 9 Gelatinase Methyl Red VogesProskauer 10 Positive 11 Negative Negative Negative 12 Citrate 13 Pectinase 14 Indole Positive Phenylalanine deaminase Utilization of 15 Negative Positive (fermentative) Positive (fermentative) 16 Glucose 17 Lactose
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