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Description: Give only typing answer with explanation and conclusion 6. What is the operon model of gene expression in bacteria? Explain how the lac operon works.

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Give only typing answer with explanation and conclusion 6. What is the operon model of gene expression in bacteria? Explain how the lac operon works.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter16: Regulation Of Gene Expression

Section: Chapter Questions

Problem 2TYK: For the E. coli lac operon, when lactose is present: a. and glucose is absent, cAMP binds and...

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Cell and Molecular Biology Subject: 1. Explain how the actions of the lac operon are different from the trp operon. 2. Why is it important for a multicellular organism to be able to turn on only some of its genes in any particular tissue or cell type?
Matching type Choices are in the picture 11. regulating elements in the operon 12. ribosome propels to the next bases 13. dozen of initiation factors involving methionly tRNA 14. eRF recognizes UGA 15. TATA box in the promoter region
explanation summary You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon?
For the E. coli lac operon, when lactose is present: a. and glucose is absent, cAMP binds and activates catabolicactivator protein (CAP). b. and glucose is absent, the level of cAMP decreases. c. activated CAP binds the repressor protein to remove it from the operator gene. d. the cell prefers lactose over glucose. e. RNA polymerase cannot bind to the promoter.
Which feature of promoters can be found in both prokaryotes and eukaryotes? GC box TATA box octamer box -10 and -35 sequences
Matching type Choices are in the picture 6. RF1 and RF2 recognize the three bases to terminate the process 7. CAAT box is found 80 nucleotides from the actual site 8. rho factor and sequence of uracil in a loop conclude the process 9. sigma factor binds to RNA polymerase in the promoter region 10. methionine is removed
Choose correct option and do explain plz. 1. The protein complex that helps RNA polymerase to cross nucleosomes during extension is:a. SWI-SNFb. poly A polymerasac. TFIISd. FACT 2. The polydenylation is carried out by:a. primaseb. polymers poly ac. a reverse transcriptased. adenylyltransferase 3. The lactose operon produces a polycistronic mRNA that includes four genes: Lacl, LacZ, LacY and LacA. True or false?
Answer the following questions: 1. Given the following genotypes, explain how the mutation (identified by a () superscript will affect E. coli grown in lactose medium. Will the lac operon be on or off? Will there be a complete set of gene products from the loc operon? What will be the implication of the missing gene product, if ever? Will the cell be able to survive in the lactose medium or not? a. p' oE Y b. p'o Y d. po v
12a. In what condition is the LacZ gene transcribed? ANSWER (in one sentence) 12b. Fill in the following table about the Lac operon (LacZ gene) Environmental Condition + Glucose and + Lactose Glucose and + Lactose - Glucose and - Lactose + Glucose and - Lactose Q 2 Body" W #3 E D Which proteins bind to the LacZ promoter? ANSWER ANSWER ANSWER ANSWER $ 4 A R F B % 5 I T MacBook Pro A U 6 G Y & 7 H Is LacZ transcribed? (Yes or No) ANSWER ANSWER ANSWER ANSWER |||| U 8 K
You decide to work on mutant strain 2. You introduce a wild-type operon into these mutant bacteria - i.e. an operons with fully functional regulatory and operon genes - to see if you can restore function. 5. When you add ethanol to these modified bacteria, the expression of genes C and D would be ( Select] 6. When you remove the ethanol, the expression of genes C and D would be ( Select )
5' 3' ORF1 ORF2 ORF3 ORF4 ORF5 1. Above are pictured 2 operons, one that includes ORF1 and OFR2 and is transcribed using the top DNA strand as the template, and the other includes ORF3, ORF4, and ORF5 and is transcribed using the bottom DNA strand as the template. 3' 5' a) Complete this diagram by using arrows (4) to indicate the position and direction of the promoter(s). It doesn't matter if you draw the arrows above or below the ORFS as long as they are pointing the right way. b) Underline and label with "RBS" the ribosome binding site(s). c) How many different RNA molecules will be made from this region of DNA? d) How many different proteins will be made from this region of DNA? e) How many promoters are found in this region of DNA? f) How many stop codons are found in this region of DNA? g) What assay would you use to investigate the protein accumulation of these ORFs?
Read aloud V Draw Highlight 2. You are studying the regulation of the lactose operon in Escherichia coli, by measuring expression of the lacZ gene (i.e production of beta-galactosidase). (a) You identify several loss-of-function mutations in which lacZ is never expressed, in the presence and absence of glucose and lactose. What components of the lac operon could be mutated to produce this phenotype? List all possibilities. (b) You identify another loss-of-function mutation with the following expression pattern: Media + glucose - lactose + glucose +lactose - glucose - lactose - glucose + lactose lacZ expression Low Low High High What components of the lac operon could be mutated to produce this phenotype? List all possibilities.