Q: What conditions are making bacteria competent to taking up a foreign plasmid? Please answer asap
A: Natural genetic competence is the ability of cells to bind and taken up exogenous DNA. It is…
Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…
A: The lysis buffer contains NaoH and SDS(sodium Dodecyl sulphate) and its used in alkaline lysis of…
Q: What method is used to transfer plasmid DNA into bacteria? Group of answer choices heat shock…
A: Plasmid It is an extra chromosomal piece of DNA found is many of the prokaryotes.
Q: what is gel electrophoresis
A: Question : What is Gel electrophoresis,?
Q: explain F' plasmid formation diagramatically
A: F’ plasmid not only has F factor but also a region of bacterial chromosome. This happens when the…
Q: Transfection efficiency refers to a) total number of transfected cells per dish O b) total number of…
A: Transfection is the process of introducing RNA or DNA intentionally in the eukaryotic cell…
Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…
A: INTRODUCTION E. coli E. coli are small gram negative motile bacilli which mainly cause urinary…
Q: how to increase the cloning procedure's plating efficiency.
A: cloning efficiency is a measure of accuracy, providing information on the number of correct clones…
Q: Explain the importance of each of the following plasmids: conjugative plasmid, R plasmid, Col…
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: Define the following terms: Bacteriophage λ DNA Plasmid Map
A: Question - Define the following terms: Bacteriophage λ DNA Plasmid Map
Q: Explain The replica plating technique?
A: Replica plating is used in the microbiological experiment. It helps in the testing of various…
Q: Why are the following reagents used? Neutralizing solution (Plasmid isolation) Isopropanol…
A: 1) Also, the neutralisation of the arrangement permits the renaturation of DNA. The enormous…
Q: 1 3. 4 Two different cultures of Escherichia coli bacteria were inoculated onto nutrient agar…
A: pGLO plasmid comprises the gene for Green Fluorescent Protein (GFP) and a gene for resistance to the…
Q: Contrast the use of plasmid vectors with that of BAC orYAC
A: Cloning vectors are also commonly called as vectors. They play an important part in molecular…
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A: DNA fingerprinting is a chemical test that shows the genetic makeup of a person or other living…
Q: Why use LC-MS for Bioanalysis?
A: Liquid chromatography -mass spectrometry is a powerful analytical tool used for separation,…
Q: explain Resistance plasmids
A: Plasmid is a genetic structure in a cell that can replicate independently of the chromosomes,…
Q: Drag and drop the appropriate text in the gaps below. Firstly, the plasmid DNA in the E. coli is…
A: Joshua Lederberg created the term "plasmid" in 1952. Plasmids are genetic elements which are…
Q: What is the purpose of arabinose in the pGLO lab, where is arabinose located, how can it be…
A: Arabinose acts as an allosteric regulator of AraC changing and tells which DNA sites it binds to and…
Q: What is cell lysis? Discuss the principles behind DNA isolation (extraction and precipitation…
A: A eukaryotic cell consists of many membrane-bound organelles. The membrane-bound organelles form…
Q: Summarize plasmid purification and PCR product cleanup for later restriction digestion, paying…
A: Plasmids are extra-chromosomal DNA that are mostly observed in certain bacteria. They are circular…
Q: What is the role of the following in the alkaline plasmid screen? G buffer (Cell Suspension…
A: G buffer (Cell suspension solution): It is a solution that consists of a mixture of Tris (pH 7.5)…
Q: After adding dna ligase to plasmid alone the sample could serve as: experimental sample…
A: Plasmids are most commonly found as small circular, double-stranded DNA molecules in bacteria. It is…
Q: Explain the features of commercially prepared plasmids ?
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Q: What is one way to determine whether a bacterial culture has receiveda recombinant plasmid?
A: Plasmids are small, extra chromosomal, and double stranded DNA structures that can replicate…
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A: Antibiotic resistance The term antibiotic resistance is made up of two words i.e. resistance(ability…
Q: .Describe the method you would use to test for the uptake of a plasmid with a DNA insert
A: Introduction: The introduction of recombinant DNA into a suitable host cell is followed by the…
Q: Generally a plasmid vector contains how many elements?a) 1b) 2c) 3d) 4
A: Deoxyribonucleic acid (DNA) is a long molecule. It contains the instructions an organism required to…
Q: State the differences between the plasmid in Figure 1 with pBR322 in terms of their sercening…
A: According to the question, we have to state the differences between the plasmid in figure 1 with…
Q: ligated into plasmid vectors and transformed into bacterial cells/colonies on agar plates. This…
A: Answer is (D) Gene Library A gene library is a collection of total genomic DNA from an organism in…
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A: 1. Immuno-electron microscopy- it is used for localising the molecules at the ultrastructural level…
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A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: Will reveal RFLP polymorphism between the DNA samples after probe detection/visualization; and
A: Restriction Fragment Length Polymorphism (RFLP) is a distinction in homologous DNA sequences that…
Q: Demonstrate a procedure that you could adopt to restrict or digest the TOL plasmid.
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A: The following terms are explained as follows:
Q: what are two differences between agarose gel electrophoresis and SDS PAGE?
A: Agarose gel electrophoresis and the SDS page technique are used to separate the molecules. The…
Q: draw structure of plasmid
A: The plasmid is the extrachromosomal deoxyribonucleic acid (DNA) that is present with the bacterial…
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Q: Use the plasmid to answer the following questions a) Digest the plasmid with EcoRI only and then…
A: The restriction enzymes are used to make nicks at certain positions on the DNA. These positions are…
Q: Polymerase chain reaction cannot amplify complete circular plasmids. True False
A: Polymerase chain reaction or PCR is a laboratory technique that is deployed to create hundreds or…
Q: The presence of colonies on the bacterial plate post transformation suggests which of the following?…
A: Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up…
Q: Describe a technique you could employ to increase the concentration of the TOL plasmid.
A: Molecular biology is the study of biological macromolecules that are involved in the various…
Q: Why do DNA polymerases fromthermophilic microbes significantly improve the PCRprocedure?
A: Step 1 :Introduction PCR has been used widely in fields such as forensics, genetic testing, and…
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A: A groundbreaking move for molecular biology has been demonstrated by the discovery of polymerase…
Give advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.
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- PCR tests are becoming more prevalent as a standard for testing for bacterial or viral disease. Describe how PCR would be used in place of a growth culture for Strep throat and why this would be advantageous.What method is used to transfer plasmid DNA into bacteria? Group of answer choices heat shock treatment biolistics electroporation microinjectionExplain why culture based biochemical and genetic testing would not be the ideal method to identify the microbe.
- Explain the steps to transform a plasmid carrying a gene of interest into a competent host cell using the heat shock treatment.Rationalize the use of all the reagents used in Agarose gel electrophoresis.Why would you use one single colony for both streaks on a plate and inoculate a culture for plasmid purification?
- Centrifugation is the first step in most fractionations, but it separates only components that differ greatly in size. Compare and contrast very high, high, medium, and low-speed centrifugations of supernatant mixtures. Define the following: SDS Polyacrylamide-Gel Electrophoresis and ImmunoprecipitationWhy is it important for a microbiology laboratory technician to use aseptic technique?discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.
- Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000What are the basic differences between the three plating methods? Fill up the following table. Streak Pour Spread Equipment/ Materials used in immobilizing and separating cells Purpose(s) (isolation, enumeration, both) Type of colonies (surface, subsurface, both) Advantage DisadvantageWhat are the advantages and disadvantages of differential centrifugation?