Give advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.

Q: Density gradient centrifugation is an inexpensive cell separation technique. Mention 2 limitations…

A: Density gradient centrifugation is a common technique for isolating and purifying biomolecules and…

Q: biotechnology lab class : 1. The bacterial streaking on antibiotic containing agar plates has been…

A: Using restriction endonucleases, a type of enzyme, restriction digestion, also known as restriction…

Q: How does the microscopy in Figure 2 show that the capsule and an S-layer can exist in the same cell…

A: Surface layer (S- layer) is the self assembled monomeric proteins forming to protect the surface of…

Q: What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for?…

A: Gelatin, also known as gelatine is a translucent, tasteless flavorless component that is often made…

Q: explain why it is not good to break sterile field  when inserting a foley catheter

A: Inserting the indwelling catheter is an invasive procedure. Sterile technique should follow while…

Q: why do some microbiologist use soil for DNA extraction

A: The DNA is exceedingly rare in the soil compared with that from plants, animals, fungi and microbes…

Q: Briefly explain Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the…

A:

Q: Draw a alternative to the Kirby-Bauer procedure.

A: Introduction: The Kirby-Bauer procedure is also known as the disk diffusion method. It is a…

Q: Counterstaining with Hematoxylin and Eosin is an important step in which of the following…

A: Counter stains are stains that are that are in different contrast color to that of the primary…

Q: How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?

A: Answer:  Rapid Strep A Test (Rapid strep test) is a test used to identify the presence of Strep A…

Q: Write the letter of your choice at the start of the sentence then support it with a short…

A: The Kirby Bauer method is an universally accepted , USFDA approved method commonly used in…

Q: Compare and contrast precipitation reactions and agglutination reactions.

A: The immune system comprises cells, tissues, organs, molecules, and various processes, provides…

Q: inical application: A 44-year-old man with HIV is receiving antibiotics through a intravenous…

A: HIV or human immunodeficiency virus is a virus causes AIDS in humans. HIV contains single stranded…

Q: 1 trpt cells trp- cells + DNase 3 2 trp cells DNA 4 TSA plate 1 trpt cells trp- cells + DNA 3 2 trp…

A: In scientific experiments, a control is an experimental group or sample that is used as a comparison…

Q: You have learned many different techniques so far this semester that allow us to identify pathogenic…

A: A pathogen is an organism that causes illness in its host, with virulence referring to the intensity…

Q: PCR is a useful tool for early diagnosis of an infectious disease.Elaborate.

A: A disease refers to an abnormal condition in the body that have negative impact on the function or…

Q: What does a white colony indicate during blue-white screening? Explain how the color is formed.

A: Gene transfer is a method in which genetic material is transferred from one bacteria to the another.…

Q: LB (Lysogeny broth) medium for the growth of E. coli,

A: Pseudomonas putida is a kind of rod-shaped, Gram-negative, non-fermenting bacterium which is present…

Q: One laboratory method to detect for COVID-19 infection is via real time RT-PCR. The process starts…

A: Real-time RT–PCR is a nuclear-derived test for detecting the presence of RNA or DNA of the pathogen…

Q: Biochemical and differential tests can be incorporated into growth media or performed on isolated…

A: It is required to provide three examples of differential test that is incorporated into growth media…

Q: Why would you use one single colony for both streaks on a plate and inoculate a culture for plasmid…

A: Introduction Plasmids are important for the transformation of bacteria. They are used as vectors and…

Q: Decide whether each of these statements is true or false, and then explain why. Laser-capture…

A: Different techniques are available to visualise a single cell from a given tissue.

Q: Assaying virus concentration requires healthy cell cultures. Give possible reasons to account for…

A: Cell cultures are more convenient and less expensive than eggs and animals, are convenient to…

Q: what are the important principle in invertase test?

A: The invertase enzyme catalyzes the hydrolysis of sucrose into its components glucose and fructose.…

Q: Explain the principle that gelatin particle agglutination test (GPAT) use to work.

A: Cymbidium mosaic virus (CymMV) is one of the most common pathogens wreaking havoc on the growth and…

Q: please provide at least one pathogen your method of analysis is used to detect.

A: Disrobe  laboratory method Western blot proteins in an aqueous sample are separated by…

Q: Draw and label the set-up of Manual paraffin wax infiltration

A: Digram of set-up of Manual paraffin wax infiltration. As per our guidelines we are not allowed to…

Q: Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the…

A: Introduction Sickle cell disorder is a rare condition in which the red blood cells of the individual…

Q: 2 3 1. zuz zuz Enz

A: ELISA stands for Enzyme Linked Immuno-Sorbant Assay. Elisa is the test which is used to measure the…

Q: Discuss and explain the results of this graph. Graph shows results of WST-1 assay. T0 ( time zero)…

A: These are the type of colorectal cancerous cell lines of human which are widely used in medical…

Q: interphase FISH is used on fixed material from a solid tumour, rather than metaphase FISH

A: Interphase FISH is a new technique introduced into the molecular cytogenetics. it is used to study…

Q: Draw a 4-inch circle. Draw out the streaking pattern used to isolate single colonies nlate

A: The streak plate method, which we used to inoculate a petri plate in Lab 2, is the most frequent way…

Q: Which of the following statements is TRUE for DNA extraction? DNA yield and purity are inversely…

A: DNA extraction is a fundamental laboratory technique used to isolate DNA from cells or tissues for…

Q: How do the following factors affect the activity of restriction enzymes? pH conditions Mg2+…

A: Restriction enzymes (restriction endonucleases) are the enzymes that can cleave at specific sites in…

Q: You are tasked with cultivating the following microorganisms: A recombinant strain of E. coli A wild…

A: Please post fourth question separately with elaborated details required in answer  For archiving E.…

Q: Describe the use of peptide nucleic acid probes in clinical microbiology

A: Peptide Nucleic Acid Probes (PNAPs) are the artificial replicas of the DNA where the deoxyribose…

Q: Demonstrate a procedure that you could adopt to restrict or digest the TOL plasmid.

A: Plasmids are extrachromosomal, circular, double-stranded, self-replicating DNA. They are naturally…

Q: Define the following terms; streak plate method, and selective media. State the purpose and…

A: Plating is a microbiological method used to separate microorganisms found in a sample. It is used to…

Q: The main of objective of using 13-Line streaking method is to get:

A: Answer: 13-Line streaking method: It is the technique of obtaining pure bacterial culture by…

Q: ELISA is a vital diagnostic tool. Reagents required for this technique include Thermostable DNA…

A: ELISA (Enzyme-linked immunosorbent assay) is a sensitive and specific laboratory technique used to…

Q: What do you mean by amphoteric substance? Give examples of substance that is/are amphoteric. What…

A: Biochemistry is the study of biochemical functions at the cellular and molecular level using…

Q: in an indirect elisa procedure what enzyme is used?

A: Enzyme-linked immunosorbent assay (ELISA) is an assay technique used for detecting and quantifying…

Q: Explain well the principle behind the streak plate method.

A: In microbiology, there are two types of cultures, pure culture and mixed culture. In pure culture,…

Q: Which of the given statements is correct in the context of visualizing DNA molecules separated by…

A: In Agarose Gel electrophoresis DNA fragments are separated under size. The negatively charged DNA…

Q: PCR tests are becoming more prevalent as a standard for testing for bacterial or viral disease.…

A: Introduction  Almost all the molecular biology techniques revolve around DNA/RNA/Proteins. DNA is…