Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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**FIGURE 3.**

**Description:**
The diagram and accompanying data display the results of genetic analysis performed on E9.5 embryos, specifically those derived from Tak1+/Δ intercrosses.

**Panel A: Schematic Diagram**
- This section illustrates a genetic construct used in the study.
- The diagram includes:
  - Restriction sites labeled as "Hd3."
  - Exon regions marked as "E1" and "E2" flanking the construct.
  - An integration sequence represented by triangles which include:
    - **β-geo:** A fusion gene used as a reporter.
    - **PLAP:** Placental alkaline phosphatase gene, indicated in purple.
  - The scale bar indicates the distance between elements is 1 kb (kilobase).

**Panel B: Gel Electrophoresis Results**
- This section shows the results of DNA extraction and gel electrophoresis:
  - Three lanes are labeled as Δ/Δ, +/Δ, and +/+.
  - Two prominent DNA bands are observed, aligning at approximately 6.7 kb and 2.7 kb.
  - These results represent different genotypes:
    - **Δ/Δ:** Homozygous deletion.
    - **+/Δ:** Heterozygous.
    - **+/+:** Wild type or non-deleted.

This analysis is instrumental in determining the genetic modifications present in the embryonic samples and verifying the successful integration and expression of experimental constructs.
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Transcribed Image Text:**FIGURE 3.** **Description:** The diagram and accompanying data display the results of genetic analysis performed on E9.5 embryos, specifically those derived from Tak1+/Δ intercrosses. **Panel A: Schematic Diagram** - This section illustrates a genetic construct used in the study. - The diagram includes: - Restriction sites labeled as "Hd3." - Exon regions marked as "E1" and "E2" flanking the construct. - An integration sequence represented by triangles which include: - **β-geo:** A fusion gene used as a reporter. - **PLAP:** Placental alkaline phosphatase gene, indicated in purple. - The scale bar indicates the distance between elements is 1 kb (kilobase). **Panel B: Gel Electrophoresis Results** - This section shows the results of DNA extraction and gel electrophoresis: - Three lanes are labeled as Δ/Δ, +/Δ, and +/+. - Two prominent DNA bands are observed, aligning at approximately 6.7 kb and 2.7 kb. - These results represent different genotypes: - **Δ/Δ:** Homozygous deletion. - **+/Δ:** Heterozygous. - **+/+:** Wild type or non-deleted. This analysis is instrumental in determining the genetic modifications present in the embryonic samples and verifying the successful integration and expression of experimental constructs.
Expert Solution
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Step 1: Explaining what is happening in the image

In this process it is clearly seen that some kind of DNA or a gene is isolated. Later on different segments of DNA are separated in the next step.

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