FIGURE 3. DNA was extracted from yolk sacs of E9.5 embryos derived from Tak1+/A intercrosses. The researcher used what methods to generate the results in B. A T E1 B SA 6.7kb- 2.7kb- Hd3 B-geo A/A+/A +/+ Hd3 Hd3 1kb IPLAP E2
FIGURE 3. DNA was extracted from yolk sacs of E9.5 embryos derived from Tak1+/A intercrosses. The researcher used what methods to generate the results in B. A T E1 B SA 6.7kb- 2.7kb- Hd3 B-geo A/A+/A +/+ Hd3 Hd3 1kb IPLAP E2
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Concept explainers
Question
Transcribed Image Text:**FIGURE 3.**
**Description:**
The diagram and accompanying data display the results of genetic analysis performed on E9.5 embryos, specifically those derived from Tak1+/Δ intercrosses.
**Panel A: Schematic Diagram**
- This section illustrates a genetic construct used in the study.
- The diagram includes:
- Restriction sites labeled as "Hd3."
- Exon regions marked as "E1" and "E2" flanking the construct.
- An integration sequence represented by triangles which include:
- **β-geo:** A fusion gene used as a reporter.
- **PLAP:** Placental alkaline phosphatase gene, indicated in purple.
- The scale bar indicates the distance between elements is 1 kb (kilobase).
**Panel B: Gel Electrophoresis Results**
- This section shows the results of DNA extraction and gel electrophoresis:
- Three lanes are labeled as Δ/Δ, +/Δ, and +/+.
- Two prominent DNA bands are observed, aligning at approximately 6.7 kb and 2.7 kb.
- These results represent different genotypes:
- **Δ/Δ:** Homozygous deletion.
- **+/Δ:** Heterozygous.
- **+/+:** Wild type or non-deleted.
This analysis is instrumental in determining the genetic modifications present in the embryonic samples and verifying the successful integration and expression of experimental constructs.
Expert Solution
Step 1: Explaining what is happening in the image
In this process it is clearly seen that some kind of DNA or a gene is isolated. Later on different segments of DNA are separated in the next step.
Step by step
Solved in 3 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education