Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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- You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions Which operons would be functional under these conditions? 2. What repressors would be made? (NOTE: name the repressors using their gene names) 3.Which repressor(s) would be made in the inactive form? 4.Which repressor(s) would be made in the active form? 5.Which repressor(s) under these conditions can bind the operator sequence? 6.Which repressor(s)under these conditions cannot bind the operator sequence?arrow_forwardIf a researcher moves the promoter for the lac operon to the region between the beta galactosidase (lacZ) gene and the permease (lacY) gene, which of the following results would you expect? A) The three genes of the lac operon will be expressed normally. B) The repressor will not be able to bind to the operon. C) The operon will still transcribe the lacZ and lacY genes, but the mRNA will not be translated. D) LacZ will not be transcribed and Beta galactosidase will not be produced.arrow_forwardThe lac operon is an inducible operon. What does this mean?arrow_forward
- The tryptophan operon of E. coli represents an example of a gene regulatory system which is: a repressible system (normally turned on, but can be repressed) a repressible system (normally repressed, but can be turned on) an activator system (normally turned off, but can be activated) an activator system (normally activated, but can be turned off) all of the abovearrow_forwardFor the trp operon, describe when it is repressed and when it is active. Compare/contrast with the lac operon. What is the CAP protein?arrow_forwardGiven the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.arrow_forward
- The following are reasons why randomized control trials (RCTs) are not used to investigate all research questions, except: a. RCTs can be expensive, which can make it difficult if funding is limited b. RCTs can be time-consuming, and it may take months and sometimes years to obtain final results c. For certain research questions and/or certain types of exposure variables, it may not be ethical to conduct an RCT d. RCTs are less valid than observational analytical study designsarrow_forwardselect all the mutations that could applyarrow_forwardThe trp operon is a repressible operon. A strain of e coli develops a mutation whereby the trp repressor protein can no longer bind to the operator. How will this effect the production of tryptophan producing enzymes? Will these bacteria be able to live in a tryptophan free media (bacteria food)?arrow_forward
- Does mutation in LacI gene that codes for the repressor protein or the mutation in the operator region cause transcription of structural genes?Will the lactose operon system will switched off ?arrow_forwardWhat is a specific region in the trp operon that you could mutate so it expresses the trp operon in conditions of excess tryptophan. Why will this mutation cause this? Operon structure: promoter, operater, structural genes promoter - DNA region that is the site of RNA polymerase sigma binding and transcription initiation operator – DNA region that influences gene expression through its interaction with a protein (transcription factor) structural genes – gene whose expression is being regulated; encodes a polypeptide or functional RNAarrow_forwardWhat is Biolistics in biology?arrow_forward
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