Explain why the process of cell fusion is necessary to produce monoclonal antibodies used for research.
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Explain why the process of cell fusion is necessary to produce monoclonal antibodies used for research.
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- Explain what a lysogen is.In relation to immunotechnology, answer the following: A- Answer the following in relation to Hybridoma technology: → What is the cancer cell line name used in hybridoma technology?Describe three examples of how antibodies are used as reagents in cell biology and molecular biology.
- Crossmatching donor red cells for a patient involves combining: Question 2 options: A) 2 drops of donor red cells with 1 drop of patient plasma B) 2 drops of patient red cells with 2 drops of patient plasma C) 1 drop of donor red cells with 2 drops of patient plasma D) 1 drop of donor red cells with 1 drop of patient plasmaWhy was a donor cell extracted from champion horseCalculate the rough cost estimation of manufacturing 250g of antibodies in 50 L bioreactor using CHO cells. 100,000 cells can produce 100 ug of antibody. Please include: How many cells that you need? Will you need to use microcarrier? How much media is required? Cost of materials: Labor: Time:
- In relation to immunotechnology, answer the following: A- Answer the following in relation to Hybridoma technology: → What is the cancer cell line name used in hybridoma technology? → Give an example of murine Monocolonal Antibody produced by hybridoma technology? B- Give ONE main difference between: Murine MABs and fully human MABs? and explain the reason behind developing different forms of Monoclonal antibodies (chimeric MABs, humanized MABs, and fully human MABs) C- You are in an internship in one major Hospital, You were in rotation with a clinical pharmacist in oncology section and you came over a breast cancer patient. The personal doctor was making a quick visit to the patient to make sure the patient is following the therapeutic protocol and the treatment is running smoothly. The doctor wrote in the prescription sheet “Trastuzumab” to be dispensed by the In Patient Pharmacy, when the clinical pharmacist saw this he advised the doctor politely to use the…Box one: • Human breast cancer cell line BT474, which overexpresses HER2, in 10 cm Petri dishes• Human HER3 ligand• Lapatinib• Lysis buffer A (containing 1% Triton and 0.1% SDS)• Lysis buffer B (containing no detergent)• Mouse anti-HER3 antibodies• Mouse anti-pY1289 of HER3, that recognises the phospho-tyrosine at position1289 of Her3• Mouse anti-actin antibodies• Horseradish peroxidase (HRP)-conjugated anti mouse IgG antibodies• SDS-PAGE Sample buffer (contains SDS, mercaptoethanol and bromophenolblue)• Molecular weight markers• SDS-PAGE gel• Nitrocellulose membrane• Phosphate buffered saline (PBS)• Blocking buffer (5%w/v BSA in PBS)• X-ray film• Luminol/H2O2 (hydrogen peroxideExplain how a capsule can allow an organism to be serum resistant and avoid phagocytosis.
- In relation to immunotechnology, answer the following: Give an example of murine Monocolonal Antibody produced by hybridoma technology?Explain how hematopoietic stem cells were experimentally shown to be both multipotent and capable of self-renewal.What is the result of the Antibody Screening Test? Based on the result of the autocontrol, it is safe to say that the antibodies which reacted with the screening cells and panel cells are?