Explain what the following factors consist of and their influence on the chromatographic separations: Theoretical number of plates (N), height of a theoretical plate (H), Linear speed, and Oven temperature.
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Q: 2. Account for the versatility of thin-layer liquid chromatography.
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Q: What is the underlying principle used in chiral chromatography to separate components of an analyte?
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Explain what the following factors consist of and their influence on the chromatographic separations: Theoretical number of plates (N),
height of a theoretical plate (H),
Linear speed,
and Oven temperature.
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- In color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?1. From spinach, the chromatographic analysis will yield two major substances: (a) B-carotene (C40H56), which will separate from the spinach mixture to form a yellow band, and (b) chlorophyll A (CSSH72MGN4OS), which will separate from the mixture to form a green band. i. Describe the characteristics and draw structures of these molecules. ii. From the spectrophotometric analysis, what wavelength (Amax) would you expect ß-carotene (C40H56) to absorb? What wavelength would chlorophyll A (CssH72MgN4O5) absorb?solve it asap. Explain what the following factors consist of and their influence on the chromatographic separations: Theoretical number of plates (N), height of a theoretical plate (H), Linear speed, and Oven temperature.
- One method for the quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is area normalization. Here, complete elution of all the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction involves dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2 and 30.2, in order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78 and 0.88, respectively.Cite the applications for utilizing molecular/size exclusion chromatography in the analysis of samples. What are some of the limitations when using this analytical method?Why does the efficiency (decreased plate height) of liquid chromatography increase as the stationary phase particle size is reduced?
- In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller moleculesDifferentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the thin layer chromatography of plant pigments? How do you say so?explain what a response ratio is in Gas Chromatography and why it might be needed to quantify compounds in a mixture?
- In stationary chromatography, the distribution (K) is the main criterion for the division of species in the sample between the two functions of the system, which factors depend onA student analyzes sample 1, and sample 2 chromatograms of a mixture containing two compounds (A + B). The following data is obtained: Sample 1: Retention time Area Height % Area 0.210 19.20 15.68 5.57 0.650 243.62 89.37 70.74 1.74 81.57 13.42 23.68 Sample 2: 0.290 64.81 49.90 12.17 0.69 11.61 4.19 2.18 1.95 456.31 43.46 85.66 Using the data from the four compounds below, answer the following questions: Compound (b.p. °C) Retention time Area Height % Area Air 0.280 14.29 8.85 2.81 Hexane (69) 0.666 93.40 33.76 18.34 Cyclohexane (80.7) 0.980 124.66 30.48 24.48 Pentane (98.4) 1.216 112.27 24.18 22.05 Toluene (110.6) 1.830 164.59 21.64 32.32