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- Arial BIUA 11 + .. | I 1 I 3 I 4 i.) Fill in the table for each of the E. coli: (0) = No Activity (+) = Basal Activity and (+++) = High Activity E. coli chromosome F' Plasmid B-gal activity? Permease activity? When When When When Glucose is Lactose is Glucose is Lactose is present present present present a.) I+ P+ O+ Z+ Y+ Inone +++ +++ b.) I^[S] P+ O+ Z+ Y+ none c.) I+ P+ O^[c] Z+ Y+ none d.) I+ P+ O- Z- Y+ none e.) I+ P+ O+ Z+ Y+ I^[S] P+ O+ Z+ Y+ f.) I^[S] P+ O+ Z- Y+ I+ P+ O^[c] Z+ Y- g.) I^[TB] P+ O+ Z+ Y 1+ P+ O^[c] Z- Y+ h.) I+ P+ O^[c] Z+ Y- I+ P+ O+ Z** Y+ i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+ Z** is a polar mutation ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of ß-galactosidase protein activity with and without lactose present, why? MacBook Air 000a. For the E.coli haploid genotype below, determine the phenotype for the following proteins. Assume that any genes or structural elements not shown are wildtype. lacls lacz lacY* lacz [ Select ] lacY [ Select ]help. 1. To produce a specialized-transducing mycobacteriophage construct, a cosmid bearing the allelic exchange substrate is electroporated in Mycobacterium smegmatis, followed by an incubation at 30°C. True/ False 2. A recombineering system involves the over-expression of recombinase proteins, which allows the use of a short DNA fragment as allelic exchange substrate, but can also be combined with the use of a mycobacteriophage construct. True/ False
- b. Cleavage with chymotrypsin produces the following fragments: Band A: CN , NLQY, GIVEQCCHKRSEY Band B: F, Y, DPTKM, IACCVRGF, RTTGHLCGKDLVNALY Cleavage with Staphilococcus aureus V8 protease produces the following fragments: Band A: GIVE, YNLQNYCN, QCCHKRCSE Band B: PTKM, RTTGHLCGKD, LVNALYIACGVRGFFYD What is the amino acid sequence of the protein? Type your responseGive me three ways that can overcome the problems during the micropropagation of jackfruit.what. is the hypothetical genetic control mechanism and iron acquisition system that C.diphtheriae. employs to acquire iron. Take into consideration that the binding constant for the corynebacteria-iron complex is 1025.
- Treated onion root tip with colchicine and untreated onion root tip model. What is the notable difference between the treated onion root tip with colchicine and untreated onion root tip.Theoretical Data Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1 Chloroform-treated lysate 1ml Dilution 10-3 10-4 10-5 0 0 0 0 0 9ml 9ml 9ml 10-1 10-² 10-3 Empty sterile tubes Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix 0.1 ml 9ml 9ml 104 10.5 // 0.1 ml 0.1 ml +0.5 ml E. coli ↓↓↓↓↓↓ Incubate O/N at 35°C, observe for plaques Molten soft agar overlay B. Quantification of the Infective E. coli other Bacteriophage Particles in the Raw Sewage Sample Volume of Stock Plated (in ml) Bottom agar No. of Plaque-Forming Units (pfu) Ave. A B 254 265 132 110 11 23What are the for phytoglycogen confirmation properties in maize can that can determine the extracted glycogen in the plants. Present it nicely.
- pls do not copy paste Explain the steps of preparing a cell culture stock from a T25 flask.APPROVED or NOT APPROVED on Bt eggplant?29 of 41 In a western blot, the primary antibody binds to the while the secondary antibody binds to the O target protein; nitrocellulose membrane O Nitrocellulose membrane; target protein O target protein: primary antibody O secondary antibody; target protein