Experimental results describing a protein's amino acid composition are useful for estimating the molecular weight (MW) of the entire protein. A quantitative amino acid analysis reveals that bovine cytochrome c contains 6% alanine (?r 89.1) by weight. Calculate the approximate molecular weight of bovine cytochrome c if there are 6 alanine residues.

Q: What is the expected molecular weight of the protein encoded by the sequence provided here:

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Q: Assume a protein is composed of 120 amino acid residues and that each amino acid can have three…

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Q: Given this: Protein Isoelectric pH Molecular weight (kDa) Ovalbumin 4.6 45 Insulin 5.4 5.7…

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Q: You are super jazzed because your protein is purified and at a concentration of 600 μg in 1.75 ml of…

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Q: You are trying to purify protein C from a mixture of proteins noted in the above Table. If you had…

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Q: exce PH pea excess [OH] Make a reasonable suggestion for which А. amino acid X might be, with either…

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Q: Saccharide X occurs in the roots of several species of plants. Deduce the structure of saccharide x…

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Q: Purification of a protein of unknown structure has been achieved. The natural protein has a…

A: Proteins are polymers of amino acids and form primary structure. It can form secondary, tertiary and…

Q: TABLE 3-1 Properties and Conventions Associated with the Amino Acids pk, values Amino acid…

A: pI refers to the isoelectric point or the pH at which the amino acid carries a positive charge and a…

Q: After determining the optimum pH, you are now tasked to determine the inhibitory effects of…

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Q: A protein sample of unknown concentration was placed in a cuvette with a path length of 1 cm and the…

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Q: Refer to the following table below to answer the succeeding questions: Protein Isoelectric pH…

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Q: whereas protein B does not absorb at 340 nm. What absorbance will be observed when light at 340 nm…

A: Given, ε (extinction coefficient) = 6440 M-1 cm-1 l (path length) = 5 mm = 0.5 cm c (concentration)…

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Q: Suppose you have a mixture of the following proteins protein A: pl = 3.5, mw = 35 kDa protein B: pl…

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Q: Solve for the isoelectric point of the following amino acids. Draw the species present before and…

A: When the pH is less than pKa, the amino acid remain full protonated. As the pH increases more than…

Q: What type of protein is included in milk? When milk sourness occurs, why does this protein…

A: Proteins are a linear chain of amino acid sequences attached together via peptide bonds. Proteins…

Q: Mr standards without with (kDa) 2-МЕ 2-МЕ 200 100 90 75 50 25 20 15 | | |I| | |||

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Q: Describe the concept of isoelectric point and calculate the PI for isoleucine?

A: Isoelectric point is the pH value at which the charge on an amino acid is zero.

Q: Does this calculation look correct? My goal isto have a target mass of 10 µg of my protein with a…

A: Yes this calculations look correct.since you want your final volume to be 30 microlitres you have to…

Q: Given this: Protein Isoelectric pH Molecular weight (kDa) Ovalbumin 4.6 45 Insulin 5.4 5.7…

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Q: When protein X is subjected to dimethylsuberimidate and SDS electrophoresis a 50 kDa band is seen;…

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Q: 1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL…

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Q: Approximate molecular weight for an unknown protein from gel-filtration experiment is 130 kDa.…

A: Given Values: Molecular weight obtained from gel-filtration experiment = 130 kDa Weight of the…

Q: Protein Isoelectric pH Molecular weight (kDa) Ovalbumin 4.6 45 Insulin 5.4 5.7 Fibrinogen 5.8 340…

A: The isoelectric point of a protein is the pH at which the net charge of the protein is zero. At this…