Elaborate on the advantages of examining colony in an agar plate.

Q: Describe how you would perform a Gram Stain. Describe how to perform the streak for isolation…

A: According to guidelines , we are supposed to attempt first three questions in case of similar…

Q: Define agar.

A: A dried jelly-like substance called agar, commonly referred to as agar-agar, is made from the red…

Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…

A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…

Q: What might happen if the Gram staining procedure is performed ona culture incubated for a little…

A: Gram staining is one of the staining methods used in laboratories to differentiate between two broad…

Q: Give two reasons for the differential staining between gram + and gram - organisms

A: Differential Staining is all those staining methods that employ more than one stain to distinguish…

Q: Sample diagram for the gram staining procedure and microscopic observation.

A: Danish Bacteriologist Hans Christian Gram in 1884 introduced differential staining techniques in…

Q: What is the primary use of agar deep, agar slant, agar plate?  explain

A: Primary use of agar deep :  • Agar deeps are used to grow bacteria which  require less oxygen . And…

Q: Write instruction to make cell culture medium using DMEM consist of 10% FBS, 1% streptomycin.

A: Introduction Streptomycin is an antimycobacterial drug which is used in the treatment of…

Q: Give the principle of acid fast staining and gram staining.

A: Microorganisms are extremely small in size and hence cannot be visualized directly. Specialized…

Q: describe the agar-overlay technique

A: Biological techniques are processes or methods for trying to study living things. They include…

Q: attern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface…

A: Slant culture : A culture made on the slanting surface of a solidified medium in a test tube that…

Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…

A: To answer this question first, we need to understand what is gram staining and what are the steps of…

Q: The factors for the manipulation of culture medium?

A: Culture medium It is also called a growth medium. It is a solid, liquid, or semi-solid medium that…

Q: In Microbiology, are there technologies that can help make bacterial culture and sensitivity done…

A: In microbiology, culture is the process of creating artificial media for microbes in order to grow…

Q: Outline the steps in gram staining

A: The common microbial technique that is used to differentiate the two large groups of bacteria…

Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…

A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…

Q: How is the correct way of incubating culture plate media? Explain why is that so.

A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…

Q: Give at least two other examples of samples that are best prepared using Smear Preparation…

A: Smear preparation technique: it is used to fix the samples onto the slides.

Q: Show a concept map about the aseptic technique on Transfer from Broth to Broth Culture, Transfer…

A: Aseptic techniques or sterile techniques are procedures that ensure proper handling and conduction…

Q: List the  steps in a Gram stain.

A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…

Q: Provide three applications of cell suspension culture

A: Suspension culture is a type of culture in which single cells or small aggregates of cells multiply…

Q: Name four bacterial diseases for which the dark-fi eld microscope is an effective diagnostic tool.

A: Dark-field microscopy is a type of microscope that illuminates unstained samples. This technique…

Q: Explain the principle of Gram staining. (You do not need to describe the procedure step by step).

A: The danish Bacteriologist Christian gram discovered this differential technique in order to…

Q: Explain the different results in the Gram stain are due to differences in the structure of the cell…

A: There are many types of bacteria and bacterial species. They are prokaryotic cell which has a cell…

Q: Explain the mechanism of Gram staining.

A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…

Q: Give the stages of gram staining

A: Gram staining also termed Gram's method is defined as a staining method for distinguishing and…

Q: Name the substance used as a medium in gel electrophoresis.

A: Electrophoresis is the process of separation of charged macromolecules under the influence of…

Q: List the reagents of the Gram stain technique in order and their general role in the staining…

A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…

Q: lain how a wire loop and wire needle is used in microbiology laboratory.

A: A laboratory dedicated to the culturing, examination, and identification of microorganisms including…

Q: laboratory? explain with diagram

A: The tissue culture is the general term for the removal of the cells, tissues or organs from an…

Q: Define the term gram staining.

A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…

Q: who introduced the use of cotton plug in microbial culture tubes.

A: Cotton plugs are utilized in bacterial specimens and culture mediums. The cotton plugs' primary role…

Q: Which laboratory procedure is represented in the diagram below? Paper towel 1) reducing the size of…

A: Often microscopic examination of a specimen involves many procedures to be followed, from slide…

Q: explain why the invention of solid culture media was of great importance to the development of…

A: Most microbiological tests rely on culture media to obtain pure cultures, grow and count microbes,…

Q: Agar is added to media.

A: Agar is obtained from algae. It is composed of agarose (polysaccharide) and agaropectin. It is a…

Q: Evaluate the following preparations in terms of providing informationon microbial size, shape,…

A: Staining is an important step in observing the microbes under a microscope. they allow to the…

Q: Describe the process of binary fission. Which methods in the lab can be used to measure growth?…

A: Binary fission can be defined as a method in which organisms duplicate their genetic material (DNA)…

Q: Need help Which of these could be used to measure the number of live bacterial cells in a liquid…

A: Bacterial cultures are used to grow the bacteria in a specific environment. A bacterial culture…

Q: What is agar and what makes it the best agent for making culture media? explain

A: Microbiology is the branch of science which deals the study of different microorganisms such as…

Q: In a tabulated form, differentiate the physical and chemical methods of sterilization.

A: The microbiological technique that is performed for the removal, killing, or deactivation of…

Q: employ a chemical (using a reagent, not a physical method) mordant? Gram stain; Acid-fast stain.…

A: The main function of modern in staining procedure is to retain the original stain or dye. It is a…

Q: explain methods for cleaning beans using flat bed screen and drum spray cleaner

A: Much of the mechanical cleaning procedures must be performed in the field for proper storage of the…

Q: Describe the difference between batch and continuous culture techniques

A: Bacteria and fungi, for example, are extremely valuable to a wide range of industries.…

Q: Briefly, in your own words, describe the steps of the gram staining procedure.

A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…

Q: Assume that you had plated your +PGLO culture on an LB agar plate. What result (number of colonies)…

A: +pGLO culture is a type of bacteria that has been genetically modified to produce a fluorescent…

Q: Describe the use of microscopy to identify microorganisms in patient specimens

A: A microorganism, often known as a microbe, is a minute living entity. Microbiology is the study of…

Q: Write the procedure for resin embedding

A: Answer: EMBEDDING = It is the process of embed a tissue for fine and regular sectioned, the most…

Q: Describe the process of Gram staining and how its results can aidthe identification process.

A: It is a differential staining which helps to distinguish between a gram-positive and gram-negative…

Q: main goal of motility agar

A: MICROBIAL GROWTH MEDIA- It is the culture media that serves as a nutrient media for the growth of a…

Q: Compare and contrast the growth of bacteria in different physical types of media (broths, slants,…

A: Growth medium, also known as culture medium involves solid, semi-solid, or liquid, medium that is…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.