During transfer of liquids using a micropipette: Would you consider your pipettes accurate ? Why?

Figure 1 show the shape of the peaks in gas chromatography that causes loss of column efficiency. Determine the type of shape and identify two possible solutions to fix it. INJ Time (min)

You want to transfer 30 µL of liquid from Tube A to Tube B. What are the steps you take to do this? Assume you have already set the volume on your pipettor and added your pipet tip to your pipettor. Lift pipet tip out of Tube B Let the plunger go to its resting position Press the plunger to the second stop Insert pipet tip into the liquid Tube A ◆ Let the plunger go to its resting position and eject the tip into a tip waste container Lift pipet tip out of Tube A Press the plunger to the first stop Insert pipet tip into Tube B

Questions about Micropipettes:    A) Imagine that you pressed your plunger to the second stop instead of the first stop when you took up water. Did you take up more or less fluid than the desired amount?   B) Now imagine that you introduced air bubbles into the pipet tip while you are taking up fluid. Did you take up more or less fluid than the desired amount?