During a pulse-chase experiment with secreted proteins, the proteins are synthesized for a short "pulse" time with radioactive or fluorescent amino acids to label the proteins. During the "chase" period, unlabeled amino acids are added, so any additional proteins synthesized are not labeled. The labeled proteins can then be monitored over time. You complete a pulse-chase experiment to monitor the movement of a protein. The protein is shown in the following digram. Its signal sequence is outlined and also indicated by the blue color in the primary protein structure.

Q: You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin…

A: 1) Pancreatic islets consist of three types of cells namely, α-cells, β-cells, and δ-cells. The…

Q: What physical parameters of a protein control its migration on electrophoresis?

A: Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules in the…

Q: While proteins are being processed how and where does this occur?

A: Protein synthesis involves two processes, they are transcription and translation. Transcription…

Q: Why would a a pure protein screening approach be used to discover a small molecule inhibitor of a…

A: INTRODUCTION A small molecule screen is a procedure in which small molecules (typically organic…

Q: Protein activity is controlled by multiple mechanisms in the cell. Briefly describe how this type of…

A: Biomolecules are organic macromolecules that play an important role in the proper functioning of the…

Q: Two proteins bind the same ligand, and the data is shown below. Select the best answer based on this…

A: A single biomolecule's 2binding affinity is the potency of the binding relationship with its ligand…

Q: The following RNA sequence represents a small messenger which can be translated in a prokaryotic…

A: The first step in translating an RNA sequence into a protein is to divide the sequence into codons,…

Q: Consider the following coding 71 nucleotide DNA template sequence (It does not contain a…

A: In cell protein formed according to the sequence on mRNA. mRNA is formed from DNA sequence by the…

Q: You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin…

A: Restriction enzymes are use to cut DNA at specific sites, known as restriction sites. The…

Q: 80 20 60 0 60 20

A: It is a way to visualise angles as a function of angles . It is the way we could identify the…

Q: Experimentally, what are some ways to investigate a protein's function/s or loss of it?

A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Q: Upstream processing is: Isolation of protein Expression of theprotein by cells Purification of…

A: Recombinant DNA technology have enabled us to use other life forms like bacteria, fungi, algae,…

Q: Polypeptides with the ER Signal Sequence (MMSFVSLLLVGILFTATEAEQLTKCEVFQ <- one-letter amino acid…

A: Proteins are synthesized in the cytosol in a process called translation. RNA polymerase and other…

Q: Define upstream processing when producing a protein and list two examples. Cite it.

A: A bioprocess is a method for producing desirable products having medicinal importance with the help…

Q: Two proteins bind the same ligand, and the data is shown below. What is the KD value for Protein B?…

A: The answer of the above question is: Correct answer not given. KD is the equilibrium dissociation…

Q: The synthesis of a protein requires that the amino acids that constitute the growing polypeptide…

A: In the intricate dance of cellular processes, protein synthesis takes center stage, orchestrating…

Q: Please explain machanism of protein folding, highlighting the role of chaperones.

A: All cellular systems are controlled by proteins. The instruction for the synthesis of proteins is…

Q: Erk and P-Erk live in two different locations within the cell even though they are just two…

A: * Mitogen activated protein kinase also called as  MAPK and  p42MAPK, and ERK2 * It is an enzyme…

Q: You are studying the regulation of the enzyme 3-phosphoglycerate dehydrogenase (3PGD). This is the…

A: Serine is a non-essential amino acid that means it is produced by our body. It is present in almost…

Q: You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin…

A: C-peptide or connecting peptide refers to the polypeptide spanning 31 amino acids that connects…

Q: How can widely separated parts of a protein interact with the spike protein

A: Spike proteins are glycoproteins that are present on the surfaces of the enveloped viruses of many…

Q: You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin…

A: The expression level of any expression vector depends on the regulatory elements like enhancer and…

Proteins

We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.  

Protein Expression

The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.