Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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During a pulse-chase experiment with secreted proteins, the proteins are
synthesized for a short “pulse” time with radioactive or fluorescent amino
acids to label the proteins. During the “chase" period, unlabeled amino
acids are added, so any additional proteins synthesized are not labeled.
The labeled proteins can then be monitored over time. You complete a
pulse-chase experiment to monitor the movement of a protein. The
protein is shown in the following digram. Its signal sequence is outlined
and also indicated by the blue color in the primary protein structure.
ER Signal Sequence
H₂NDM
U
O
VOGVEXDXDXE
Primary Protein Structure
B28
A7-87
B26
B8
B12
A19
B24
B23
B11
B5
A16 A13
A6-A11
B15
B20
Folded Protein
(Ball and Stick)
B1
A20-B19
B-chain
A chain
Folded Protein
(Ribbon diagram and space
filling)
endoplasmic reticulum → transport vesicle → Golgi → cytosol → exocytosed
endoplasmic reticulum → transport vesicle → Golgi → transport vesicle →
exocytosed
endoplasmic reticulum → transport vesicle → Golgi → cytosol → embedded in
the plasma membrane
endoplasmic reticulum → transport vesicle → Golgi → transport vesicle →
embedded in the plasma membrane
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Transcribed Image Text:During a pulse-chase experiment with secreted proteins, the proteins are synthesized for a short “pulse” time with radioactive or fluorescent amino acids to label the proteins. During the “chase" period, unlabeled amino acids are added, so any additional proteins synthesized are not labeled. The labeled proteins can then be monitored over time. You complete a pulse-chase experiment to monitor the movement of a protein. The protein is shown in the following digram. Its signal sequence is outlined and also indicated by the blue color in the primary protein structure. ER Signal Sequence H₂NDM U O VOGVEXDXDXE Primary Protein Structure B28 A7-87 B26 B8 B12 A19 B24 B23 B11 B5 A16 A13 A6-A11 B15 B20 Folded Protein (Ball and Stick) B1 A20-B19 B-chain A chain Folded Protein (Ribbon diagram and space filling) endoplasmic reticulum → transport vesicle → Golgi → cytosol → exocytosed endoplasmic reticulum → transport vesicle → Golgi → transport vesicle → exocytosed endoplasmic reticulum → transport vesicle → Golgi → cytosol → embedded in the plasma membrane endoplasmic reticulum → transport vesicle → Golgi → transport vesicle → embedded in the plasma membrane
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