Draw a schematic diagram of a double beam fluorescence spectrophotometer.          Explain the design of a spectrofluorimeter for high throughput measurement.        Write down the name of two source lamps used in the fluorescence   spectrophotometer? How do you calibrate a fluorescence spectrophotometer?.

Q: Why is it important to use standards or to develop a standard curve in spectrophotometry?

A: Spectrophotometry is the analytical technique by which the character of a substance is measured…

Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.

A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…

Q: how do you purify a protein for single particle cryo-electron microscopy?

A: We would require a protein sample with more than 99% purity in order for it to qualify as a sample…

Q: What are the main instrumentation components difference between Uv-visible and Fluorescence…

A: Spectroscopy :-  Spectroscopy is basically an association of electromagnetic radiation with atoms ,…

Q: What is gel electrophoresis and why is it important? Write in detail.

A: Electrophoresis is the technique that is used to separate biomolecules that have a charge in them.…

Q: Based on this figure, what is the minimum actual size (in mm) of the Volvox sp. provided that you…

A: Volvox is the species of algae that belongs to class chlorophyceae. In this species, cells lives in…

Q: How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a…

A: A spectrophotometer is an instrument that is used to measure the intensity of light absorbed by the…

Q: Briefly explain how the API (Analytical Profile Index) system works.

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: Suppose you were in a research lab in Romania and the lab is equipped with several…

A: Proteins are biomolecules that play a variety of roles in living systems. They include catalysis,…

Q: Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement…

A: An absorption spectrophotometer is defined as a device that is used to calculate the absorbed light…

Q: How can you tell if the current or the gel electrophoresis is already running and what are the…

A: Gel electrophoresis is the process where DNA, RNA, and protein fragments get separated depending on…

Q: At what wavelengths should the absorbances be read to examine the purity of a DNA sample with a…

A: A spectrophotometer is a tool that measures the number of photons (light energy) absorbed after…

Q: What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and…

A: Introduction: Spectrophotometry is a method utilized for the evaluation and portrayal of drab mixes…

Q: The following is an UV-Vis schematic diagram. Based on this diagram, answer the following questions:…

A: UV-vis spectrophotometer A spectrophotometer is an instrument or apparatus that is used in the…

Q: What are the light sources used in UV-vis spectrophotometry.

A: In  case of visible spectrophotometers, the wavelengths measured are in the visible light range…

Q: What wavelength is most suitable for quantitative analyses for an analyte exhibiting the UV-Vis…

A: Quantitative analysis is the method of determination of concentration of an analyte where teh amount…

Q: Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2.…

A: Answer: Photometer : It is the instrument which is used to measure the intensity of a beam of light.…

Q: Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.

A: Proteins are defined as the biological molecule that are produced by the building blocks amino…

Q: Name some variables that would likely affect polarimetry results.

A: Polarimetry is a laboratory technique used to specify the angle at which polarized light has been…

Q: What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x…

A: Given: Objective lens 1 magnification= 1000x DFV1 = ? Objective lens 2 magnification= 400x DFV2 =…

Q: the figure shows the result of the spectral analyzer for paper spectrophotometer without any…

A: Paper spectrophotometry is used in printing industry. In the printing industry, a spectrophotometer…

Q: Discuss the concept of spectrophotometry, colorimetery, and turbidimetry. Maximum of 150 words

A: Spectrophotometry is a part of electromagnetic spectroscopy worried about the quantitative…

Q: What is safe view dye for? Compare it with Ethidium Bromide.

A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…

Q: Young's double slit experiment is one of the quintessential experiments in physics. The availability…

A: To calculate the fringe separation between the second-order and central fringe in Young's…

Q: Here are the materials and method for the basic spectrophotometer experiment Materials: 1. Paper…

A: A spectrophotometer is a device used to measure the spectrum of visible light. The wavelength and…

Q: Why does one see methyl orange as orange in color? What blank would be used to standardize this…

A: 1. Methyl orange is a pH indicator frequently used in titration because of its clear and distinct…

Q: Define each of the following terms:  A) What is resolution and how is resolution related to the…

A: According to the question, we have to provide the solution of the questions one by one. These…

Q: What is the reason for the decrease in the ABS value over time when measuring in the…

A: A spectrophotometer is a device used in an analytical chemistry lab to measures the amount of light…

Q: Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc).…

A: Electrophoresis is the migration of charged particles under the influence of an electric…

Q: ssume that you have an “X” solution that you do not know its concentration. But you have other X…

A: Absorbance of X solution Concentration Absorbance       0.025   0.22 0.05   0.44 0.1…

Q: List four reasons for the development of pulsed field gel electrophoresis

A: Electrophoresis is a general term that describes the migration and separation of charged particles…

Q: Draw Fluorescence Resonance energy transfer(FRET) and describe how it works.

A: Introduction :- The normal fluorescence microscopy techniques works on the principle of the…

Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…

A: Fluorescence quenching is a method that diminishes the fluorescence force of an sample. A number of…

Q: Please search on-line and find a manufacturer's data sheet for a transducer or sensor used in a…

A: The primary sensors are used in medical devices for pressure, force, airflow, oxygen, pulse…

Q: Describe step by step process of: a. Ion-exchange Chromatography b. Hydrophobic Interaction…

A: Introduction: Chromatography is a physical method for the separation of compounds. Tswet, a Russian…

Q: In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used…

A: UV/Visible Spectrophotometry detects the absorbance of light in the UV/Visible range of the…

Q: What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail…

A: Errors and limitations of gel electrophoresis.

Q: Describe how you would prepare the following samples for analysis by a spectroscopic method. You…

A: Spectroscopic method or techniques is the process that recruit the light to link with matter. This…