Draw a schematic diagram of a double beam fluorescence spectrophotometer.          Explain the design of a spectrofluorimeter for high throughput measurement.        Write down the name of two source lamps used in the fluorescence   spectrophotometer? How do you calibrate a fluorescence spectrophotometer?.

Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.

A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…

Q: how do you purify a protein for single particle cryo-electron microscopy?

A: We would require a protein sample with more than 99% purity in order for it to qualify as a sample…

Q: Describe the mechanism of SERS( Surface Enhanced Raman Spectroscopy).

A: Introduction: Surface-enhanced Raman spectroscopy (SERS) is a strong microscopic observation…

Q: What are the main instrumentation components difference between Uv-visible and Fluorescence…

A: Spectroscopy :-  Spectroscopy is basically an association of electromagnetic radiation with atoms ,…

Q: Based on this figure, what is the minimum actual size (in mm) of the Volvox sp. provided that you…

A: Volvox is the species of algae that belongs to class chlorophyceae. In this species, cells lives in…

Q: Objective lens X has a limit of resolution equal to 0.2 mm while objective lens Y has 30 µm. a.…

A: * object lens is the optical element which gathers light from object and focuses light rays to…

Q: (a) You decide to purify the 5-HT 2A receptor. The protein has a predicted molecular Mass of 46.54…

A: Formation of the peptide bond neutralises any charge due carboxyl group, therefore the net charge on…

Q: Why is the wavelength of the light that is released in spectrophotometric analysis longer than the…

A: Some molecules absorb light at a particular wavelength and emit light in higher wavelength and this…

Q: Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer…

A: UV-Vis Spectroscopy is also known as Spectrophotometry and is a quantitative technique to determine…

Q: Briefly explain how the API (Analytical Profile Index) system works.

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: WHAT ARE THE Steps in spectrophotometer

A: -Spectrophotometer is an optical instrument which is used to measure the concentration of a known…

Q: What is the meaning of the different values given engraved on each objective?

A: Microscopes are used in viewing microscopic organisms, including bacteria, parasites, etc., and also…

Q: Why is spectrophotometric analysis light longer than fluorescent molecule activation light?

A: Spectrophotometric analysis is a technique used to measure the amount of light absorbed or…

Q: What are the light sources used in UV-vis spectrophotometry

A: Spectrophotometer is an instrument used for measuring the concentration of solutes in solution by…

Q: Suppose you were in a research lab in Romania and the lab is equipped with several…

A: Proteins are biomolecules that play a variety of roles in living systems. They include catalysis,…

Q: What is known as electropherogram ?

A: An analytical procedure that is used for the separation of fragments of DNA or RNA, in terms of…

Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?

A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…

Q: Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement…

A: An absorption spectrophotometer is defined as a device that is used to calculate the absorbed light…

Q: At what wavelengths should the absorbances be read to examine the purity of a DNA sample with a…

A: A spectrophotometer is a tool that measures the number of photons (light energy) absorbed after…

Q: What are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference…

A: Spectrophotometric analysis has been widely used in biomedical science to determine the…

Q: What is FRAP (Fluorescence Recovery After Photobleaching)? Draw a graph of fluorescence versus time…

A: FRAP or Fluorescence Recovery After Photobleaching It is a method that was developed in 1976 by…

Q: Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a…

A: Resolving power:  An instrument's ability to resolve two points that are near to one another is…

Q: What is a Spectrogram

A: Spectrogram is a visual way of viewing the spectrum of frequencies of signal that varies with time.

Q: What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and…

A: Introduction: Spectrophotometry is a method utilized for the evaluation and portrayal of drab mixes…

Q: The following is an UV-Vis schematic diagram. Based on this diagram, answer the following questions:…

A: UV-vis spectrophotometer A spectrophotometer is an instrument or apparatus that is used in the…

Q: Explain the difference in the working principles of Dark-field and Light-field microscopes using pKk…

A: Microscope: It is an optical instrument that uses a lens or a combination of lenses to produce…

Q: the Marquis test was used on a sample suspected to contain amphetamine in a “field” sample, so the…

A: The colourimetric analysis involves the determination of a chemical substance (inorganic and…

Q: You may want to use this resource for this problem. If you do, submit the output along with your…

A: The confocal microscope is an advanced imaging technique that allows for high-resolution and…

Q: Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2.…

A: Answer: Photometer : It is the instrument which is used to measure the intensity of a beam of light.…

Q: Provide a list of the names of the devices and technologies used in Dynamic Light Scattering applied…

A: Dynamic Light Scattering (DLS) employs a measurement method to identify the particle size in…

Q: Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.

A: Proteins are defined as the biological molecule that are produced by the building blocks amino…

Q: list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet…

A: Endospores are structures produced within certain bacterial cells that allow them to survive harsh…

Q: Name some variables that would likely affect polarimetry results.

A: Polarimetry is a laboratory technique used to specify the angle at which polarized light has been…

Q: What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x…

A: Given: Objective lens 1 magnification= 1000x DFV1 = ? Objective lens 2 magnification= 400x DFV2 =…

Q: the figure shows the result of the spectral analyzer for paper spectrophotometer without any…

A: Paper spectrophotometry is used in printing industry. In the printing industry, a spectrophotometer…

Q: what is the proper position for using an incentive spirometer?

A: Lungs are the important organs of the respiratory system allowing filtration of air that is inhaled…

Q: Discuss the concept of spectrophotometry, colorimetery, and turbidimetry. Maximum of 150 words

A: Spectrophotometry is a part of electromagnetic spectroscopy worried about the quantitative…

Q: Look for recent (2017 to 2022) scientific papers, stories, or features that demonstrate the use of…

A: Spectroscopy is an analytical method that is used for the detection of unknown compounds. In the…

Q: What is safe view dye for? Compare it with Ethidium Bromide.

A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…