Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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...... doesn't need during vitro transcription
dna template
s
dNTPs
rns polymerase
all
none
Expert Solution
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Step 1
In vitro transcription is a straightforward strategy that takes into account format coordinated union of RNA molecules of any sequence from short oligonucleotides to those of a few kilobases in μg to mg amounts.
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- E D ot OH ON O Replication R SORO Transcription ORNA processing Translation Gene expression 657 F Q Search % T G LOCLE Y F7 & 87 F8 U 99+ 8 F9 53879 10 F10 O 2 H J K L CV BN MO < F12arrow_forwardAlternative splicing Template strand S F yIGUide1,mq00:c-00: Replisome Transforming principle Origin of replication (or)eleb al msxS ain Coding strand Transcription factors Leading strand Single nucleotide polymorphism Okazaki fragment Telomerase M Nucleoside RNA Polymerase I RNA Polymerase II RNA Polymerase IIIon & of qu 9ven UoY Insertion mutagenesis Spliceosome Transcription Unit SNP Reverse transcriptase 1 Seminal work by Oswald Avery and colleagues demonstrated that DNA is what Frederick Griffiths called this etniog OS dotsM bioW 1-2kb of newly synthesized DNA strands are called this ainiog PS Assembly of the replisome is an orderly process that begins at these precise sites Snoiteeu 4 Transcribes ribosomal RNA genes in eukaryotes 5. A large nucleoprotein complex that coordinates activity at the replication fork Single base pair differences between homologous genomic regions isolated from different members of a population Complex of proteins and snRNAs catalyzing the removal of…arrow_forwardWhich of the following is correct about transcription? options: The reaction is driven forward by the hydrolysis of pyrophosphate PPi -> 2Pi RNA polymerase must be able to move from nucleotide to nucleotide. Once polymerase has finished adding nucleotides, the DNA-RNA hybrid dissociates and the DNA double helix reforms. All of the abovearrow_forward
- If Avery, MacLeod, and McCarty had determined that the transforming molecule was RNA, what experimental results would have been observed? O S cell DNA extracts treated with RNase would lyse the nucleus O S cell DNA extracts treated with RNase would not transform the R cells S cell DNA extracts treated with DNase would not transform the R cells O S cell DNA extracts would not transform the R cells at all O none of the answers are correctarrow_forwardWhich process is illustrated in the diagram? T 3' 5. 3' Replication Transcription ORNA processing Translation Gene expression 5' 3' inm 13' 15'arrow_forwardPolymerase movement RNA POLYMERASE -Nucleotide being added to the vijend of the RNA RNA NTPS RNA-DNA hybrid region 32. Refer to the transcription bubble diagram (above) to fill in the blanks. 5'- 3'- Template strand/Sense strand Coding strand/Antisense strand Region of DNA unwinding Region of DNA rewindingarrow_forward
- To initiate transcription. binds to a specific v called the , located at the 5' end of a gene. DNA polymerase RNA polymerase leading strand A Moving to another q lagging strand sponse. primer primase promoter DNA sequence RNA sequence enhancerarrow_forwardWhich of the following statement(s) correctly describe(s) telomerase? Select all that apply. You may select multiple options. ✔Telomerase synthesizes DNA from an RNA template. O All of these options O Telomerase extends the lengths of telomeres of linear chromosomes in eukaryotes. O Telomerase unwinds the DNA duplex at telomeres. Telomerase serves as a primer for DNA synthesis at telomeres. O Telomerase has 5'-to-3' exonuclease activity.arrow_forwardBecause of the way the lagging strand of an DNA molecule is replicated, the 3' end of the molecule is usually not completely copied into a double-stranded molecule. Why doesn't this normally affect cell function? the genes in this area are duplicated elsewhere on the chromosome DNA is not important for normal cell function there are only genes on the other strand of DNA - not on the one ending in 3' the telomere region does not contain genesarrow_forward
- Some events that may occus during transcription 1. RNA polymerases detach 2. DNA polymerases detach 3. DNA polymerases proofread 4. RNA double helix is unzipped 5. DNA double helix is unzipped 6. DNA double helix forms again 7. Free nucleotides in the nucleus bond to exposed nucleotides of the senes strand 8. Free nucleotides in the nucleas bond to eposed nucleotides of the sense antisense strand 9. RNA ligase fuses the Okazaki fragments 10. RNA polmerase binds to the sense strand 11. DNA polymerase binds to the sense strand 12. RNA polymerase binds to the antisense strand 13. DNA polymerase binds to the antisenes strand List in sequences the events involoved in DNA transcription numbers above are ______, _______, ________, ______, and ________arrow_forwardMain... My Drive - Google... 8. Which statement/s about RNA is/are true? (Select all that apply.) * RNA is made through the process of translation RNA is made through the process of transcription and translation RNA is made through the process of transcription Some types of RNA just exist in the nucleus without ever needing to be made 9. Identify the TRUE statement about the process of transcription: * RNA polymerase requires an RNA primer molecule before it can begin transcripti DNA is not needed for the process of transcription. RNA polymerase binds to a promoter region in the DNA to initiate transcription. During the elongation phase, RNA transcripts are built from 3' to 5'. 10. Which is correct? * Translation occurs in the nucleus; transcription occurs in the cytoplasm Transcription occurs in the nucleus; translation occurs in the cytoplasm Both transcription and translation take place in the cytoplasm Both transcription and translation occur in the nucleus 1arrow_forwardWhich repair process(es) use(s) a DNA polymerase? Select all that apply. V base excision repair O photoreactivation repair O DNA Proofreading nucleotide excision repair homologous recombination repair (synthesis-dependent strand annealing)arrow_forward
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