DNA solution is viscous because of the nature of chemical substance that can intercalate into the DNA helix. An example of such substance is acridine orange. experiments revealed that acridine orange causes an increase in the viscosity of DNA solution.how would you account for this effect?
Q: Explain how an agarose gel can separate DNA fragments of different lengths.
A: Agarose gel electrophoresis is one of the important molecular techniques used for the separation of…
Q: The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 10 9 D. In your…
A: Hi! As you have posted multiple questions, I will be answering the first and third question for you.…
Q: a) In a DNA double helix, why doesn't an A or T form two hydrogen bonds (out of the three possible)…
A: Since you have asked multiple questions with multiple parts, we will answer only the first three…
Q: Consider the structure of double-stranded DNA. When DNA is placed into distilled water, it…
A: Deoxyribonucleic acid (DNA) is defined as an organic chemical that will contain all the genetic…
Q: You are given two solutions containing different purified DNAS. One is from the bacterium P.…
A: In biology and genetic science, GC-content (or G-cytosine content) is that the proportion of…
Q: DNA molecules of different sizes are often separated with the use of a technique called…
A: Electrophoresis is a technique that is used to separate molecules based on their sizes.
Q: DRAW A DNA STRAND WITH 10 ADENINE BASES FOLLOWED BY 10 CYTOSINE BASES. IF THAT SAME STRAND BONDED TO…
A: Deoxyribonucleic acid (DNA) is a double helical structure, which is composed of nucleotides. The two…
Q: perform an experiment to determine the complete melting profile of a relatively long DNA duplex with…
A: Melting temperature is the temperature at which half of the double stranded DNA gets melted out…
Q: To maintain uniform diameter of the DNA double helix, only purines base with each other via two…
A: DNA is the fundamental structure that carries all the information of a living thing. In most cases,…
Q: The double helical structure of DNA is intrinsically unstable and easily dissociates to form two…
A: DNA is deoxyribose and it has a two-strand DNA strand, each stand is made up of a nucleotide that…
Q: DNA-driven DNA synthesis forms hydrogen bonds with antisense bases. True or False?
A: Antisense is the non-coding DNA strand of a gene. In the cell, the antisense DNA fills in as a…
Q: The compound known as nitrous acid is a reactive chemical that replaces amino groups (−− NH2) with…
A: The given compound nitrous acid substitutes the cytosine(C) with uracil (U) and adenine (A) with…
Q: Which of the following relations will be true for the percentage of bases in double-stranded DNA? a.…
A: The deoxyribonucleic acid is the genetic material that is passed from one generation to another…
Q: 10
A: DNA replication is a process by which new copy of DNA molecules are made by DNA polymerase.
Q: What is the contribution of Van der Waals forces in the stability of the DNA the double helix…
A: Introduction: DNA is a hereditary material present in the living cell. James Watson and Francis…
Q: the human immunodeficiency virus HIV uses RNA rather than DNA to encode genetic information. During…
A: Answer: Introduction: The human immunodeficiency viruses (HIV) are the type of a subgroup of…
Q: The DNA polymerase cannot start making a new DNA polymer out of nothing, it needs preexisting…
A: DNA molecules makes their own identical copies by a process known as DNA replication. It takes…
Q: How Can Fragments of DNA Be Separated From One Another? Agarose gel electrophoresis is a procedure…
A: Note- as we are allowed to only 3 subparts of a question, i can provide answers for first three…
Q: In a DNA double helix, why doesn't an A or T form two hydrogen bonds (out of the three possible )…
A: The DNA (deoxyribonucleic acid) is composed of nucleotides that are in turn made up of nitrogenous…
Q: Watson and Crick used an approach called model building to deduce the structure of the DNA double…
A: DNA structure was not clear before 1950s. At the beginning of 1950s, James Watson and Francis Crick…
Q: Biochemist Erwin Chargaff was the first to discover that, in DNA, [A] = [T] and [G] = [C]. These…
A: In a double-stranded stranded DNA, the amount of purine nucleotide is equivalent to the number of…
Q: You have discovered an enzyme secreted by a particularly virulent bacterium that cleaves the C2′—C3′…
A: Introduction: DNA, also known as deoxyribose nucleic acid is made up of nucleotides which comprises…
Q: The Watson and Crick model of DNA structure shows the following EXCEPT A the turn of the DNA helix…
A: Watson and Crick proposed a specific DNA structure called the 3-dimensional DNA. DNA is comprised of…
Q: -rich DNA is denser than -rich DNA because has a higher molecular mass than while occupying the same…
A: DNA is made up of nitrogenous bases (adenine, guanine, cytosine, and thymine). DNA is usually a…
Q: If you analyze a double-stranded DNA molecule and find that 15% of all the nucleotide bases are…
A: According to Chargaff's rule, [A]+[G]=[C]+[T] where A - adenine G - Guanine C- Cytosine T- Thymine
Q: Explain , What holds the sides of the DNA ladder together?
A: DNA is composed of four nitrogenous bases (A, T, G, C) pentose sugar, and a phosphate group. The…
Q: Which of the following is true about the denaturation of double-helical DNA? A. Denaturation…
A: The hydrogen bonds between DNA strands weaken and eventually break when the temperature of a DNA…
Q: what happens to the intactness of DNA if extracted DNA fibers were placed in buffer of pH 3?
A: Extracted DNA is generally stored in neutral pH.
Q: DNA contains thymine rather than uracil. Why is the presence of thymine in DNA advantageous? What…
A:
Q: Supercoiled DNA migrates through an agorose gel faster than similarly sized linear DNA? True or…
A: According to bartleby guidelines, when multiple questions are posted. We are allowed to answer the…
Q: When a double-stranded DNA molecule is exposed tohigh temperature, the two strands separate, and…
A: Hi! Thanks for your question. I am answering only the first question, as the image is not given in…
Q: The two strands of a DNA double helix can be separated by heating. If you raise the temperature of a…
A: A double-stranded DNA is joined together by a bond and seems to be a twisted ladder. Which is…
Q: Which of the following subunits of DNA polymerase has proofreading ability?
A: Introduction: The hereditary material which transfers from parents to offspring is DNA. It is stored…
Q: olds the two strands of a DNA double helix together?
A: Deoxyribonucleic acid (DNA) is a genetic material and it carries genetic information from one cell…
Q: Why evenly spaced sequence in chromatogram is an indication of a good DNA chromatogram?
A: The characteristics of an evenly spaced sequence are:- Evenly-spaced peak, each being…
Q: What factors promote the fidelity of replication during the synthesis of the leading strand of DNA?…
A: Factors which promote the fidelity of replication are DNA polymerase enzyme ,mismatch repair system…
Q: тyou DNA, a. Will the melting temperature be the same? Why b. Will the annealing temperature be the…
A: DNA, or deoxyribonucleic acid, is the molecule that contains the genetic information of an organism.…
Q: When DNA is heated, it denatures; that is, the strands separate because hydrogen bonds are broken…
A: Introduction: DNA is the type of nucleic acid that is present in the nucleus of the cell. It is the…
Q: What are the qualities of an appropriate sequence in DNA chromatogram? Why?
A: Introduction: DNA stands for deoxyribonucleic acid. It's made up of nucleotides, which are…
Q: ich of the following pieces of DNA is going to be easier to separate into single stranded molecules…
A: A nucleotide is 2 chemical bases bonded to 1 another forming a "rung of the deoxyribonucleic acid…
Q: Which of the following DNA strands below is expected to have the highest melting temperature?
A: DNA is the genetic material in living beings. It can be seen both in prokaryotes and eukaryotes. The…
Q: . In a supercoiled DNA, a stretch of about 20 base pairs changes from the B form to the Z form. What…
A: DNA is majorly classified into three types as B, A and Z. In B-DNA, there are 10 bases in one…
Q: True or false: During DNA replication, the DNA template is read 3 ́ to 5 ́ by a DNA polymerase, as…
A: DNA replication is a biological process in which two identical copies of DNA are formed during cell…
Q: A double-stranded DNA molecule (B-DNA) is 100,000 base pairs long. How many complete turns are there…
A: The DNA double helix biopolymer of nucleic acid is held together by nucleotides which base pair…
Q: Which of the following is not part of the DNA backbone?
A: Deoxyribonucleic acid is a molecule with two long strands coiled around a common, imaginary, central…
Q: Let’s say that you want to find out the difference in nucleotide sequence among two DNA strands, one…
A: DNA sequencing is a technique used to study the nucleotides of DNA. It is used to determine the…
Q: Why does increasing salt concentration increase the melting temperature of DNA?
A: DNA is a type of nucleic acid in which two antiparallel polynucleotides are coiled around a central…
DNA solution is viscous because of the nature of chemical substance that can intercalate into the DNA helix. An example of such substance is acridine orange. experiments revealed that acridine orange causes an increase in the viscosity of DNA solution.how would you account for this effect?
Step by step
Solved in 2 steps
- DNA molecules of different sizes are often separated with the use of a technique called electrophoresis . With this technique, DNA molecules are placed in a gel, an electrical current is applied to the gel, and the DNA molecules migrate toward the positive (+) pole of the current. What aspect of its structure causes a DNA molecule to migrate toward the positive pole?2) When DNA is placed in distilled water, which is pH 7.0, it denatures (i.e., the two strands separate). The pH inside a cell is generally 7.2-7.5, depending on the organism, but DNA is generally double-stranded under physiological conditions. Briefly explain, in your own words, why DNA denatures when placed in distilled water but not when it is inside a cell. [Reminder: the pKa for the phosphate groups in the sugar-phosphate backbone of a strand of DNA is 2.14]If a DNA double helix contains 28% T nucleotides, then what is the percentage of A nucleotides?
- TRUE OR FALSE a) The 2 chains composing one double helix run in opposite directions; they are antiparallel (one is 5’->3’ and the other 3’->5’). b) DNA molecules can perform their function in replication and transcription as long as the hydrogen bonds between the bases remain intact.DNA has unique properties that allow it to accurately retain genetic information, even after multiple rounds of replication. One aspect of DNA that allows it to accurately store genetic information is the base pairing from Chargaff's first rule of the four nucleotide bases. If the A content of a DNA molecule is 18%, what are the percentages of the remaining bases? T = G = C = % % %The two sides of the DNA double helix are connected by pairs of bases (adenine, thymine, cytosine, and guanine). Because of the geometric shape of these molecules, adenine bonds with thymine and cytosine bonds with guanine. The figure below shows the bonding of thymine and adenine. Each charge shown is +e or - e, and the H-N distance is 0.110 nm. (a) Calculate the net force that thymine exerts on adenine. Is it attractive or repulsive? To keep the calculations fairly simple, yet reasonable, consider only the forces due to the O- H-N and the N-H-N combinations, assuming that these two combinations are parallel to each other. Remember, however, that in the O-H-N set, the O- exerts a force on both the H+ and the N-, and likewise along the N–H-N set. (b) Calculate the force on the electron in the hydrogen atom, which is 0.0529 nm from the proton. Then compare the strength of the bonding force of the electron in hydrogen with the bonding force of the adenine-thymine molecules (H (H (H)…
- What is the concentration of a DNA solution that absorbs 0.812 and 0.463 at 260 and 280 nm, respectively? Is the DNA solution considered to be good quality? Why or why not?The two sides of the DNA double helix are connected by pairs of bases (adenine, thymine, cytosine, and guanine). Because of the geometric shape of these molecules, adenine bonds with thymine and cytosine bonds with guanine. The figure (Figure 1) shows the thymine-adenine bond. Each charge shown is ±e, and the H−N distance is 0.110 nm . Calculate the net force that thymine exerts on adenine. To keep the calculations fairly simple, yet reasonable, consider only the forces due to the O−H−N and the N−H−N combinations, assuming that these two combinations are parallel to each other. Remember, however, that in the O−H−N set, the O− exerts a force on both the H+ and the N−, and likewise along the N−H−N set. Express your answer in newtons. Is the net force attractive or repulsive?A double-stranded DNA molecule is 1 cm long, and the percentage of adenine is 15%. How many cytosines does this DNA molecule contain?
- The complementary strands of DNA in the double helixare held together by hydrogen bonds: G ≡ C or A = T.These bonds can be broken (denatured) in aqueous solutions by heating to yield two single strands of DNA(see Figure 1-13a). How would you expect the relativeamounts of GC versus AT base pairs in a DNA doublehelix to affect the amount of heat required to denatureit? How would you expect the length of a DNA doublehelix in base pairs to affect the amount of heat requiredto denature it?You are supplied with the following information about a DNA molecule: The molecular weight of a typical nucleotide is 660 Da. The linear density of double stranded DNA is 4 x 106 Da per micrometer. There are 10 base pairs in one complete turn of the double helix. Answer the following questions about a DNA molecule with a length of 3.3 micrometer and a base composition which contains 20% of the base adenine (A). (a) How many phosphor atoms are present in this DNA molecule?(b) How many full turns of the DNA helix are there in this molecule?(c) What is the percentage of guanine bases in the DNA molecule?(d) How many hydrogen bonds are there in the molecule?(e) How many phosphodiester bonds are there in the molecule?( f ) What is the length in basepairs of a DNA molecule that contains 606 full turns of the double helix?If DNA concentration is 2 grams, what would be the molar concentration of human DNA in a human cell?