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Discuss how you would confirm that a bacterium isolated from
soil piling can utilize
nitrogen source.
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- You are characterizing a newly discovered organism . You have noticed that the organism grows on two carbon sources - sucrose and mannitol . Whenever you add both sugars to the medium , sucrose will be completely metabolized before mannitol . a ) What is this growth pattern called ? b ) Describe a possible growth curve of sequential metabolism of the sugars .Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli b. Pseudomonas aeruginosMacConkey agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. Define what type(s) of media MacConkey agar is, using as many terms in the answer list as are applicable.
- Give the components, properties and special considerations in preparation (if there's any) on the following media 1. MacConkey Agar 2. EMB Agar 3. HEA 4. SSAIs the eosin methylene blue agar? (EMB) a complex or defined medium? Explain based on its composition. What media is MSA explaining based on what kind of Organisms it allows to growMannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?
- Why is agar ideal for microbial cultivation?A sharp growth line in the SIM media is an indication of what characteristic? A) Hydrogen sulfide gas production B) Tryptophan breakdown C) Indole production D) Non-motileChoose for the organisms below, depending on the incubation condition and media whether they will grow or not 1.Haemophilus influenzae in HBA CO2 2.Haemophilus influenzae in HBA anaerobic 3.Haemophilus influenzae in Chocolate agar CO2 4.Haemophilus influenzae in MacConkey 5.Clostridium perfringens in HBA CO2 6.Clostridium perfringens in HBA anaerobic 7.Clostridium perfringens in Chocolate agar CO2 8.Clostridium perfringens in MacConkey 9.Escherichia coli in HBA CO2 10.Escherichia coli in Chocolate agar CO2 11.Escherichia coli in MacConkey 12.Escherichia coli in HBA anaerobic.
- A culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting substrate is inoculated with 10g of bacteria in a batch fermenter. The yield of biomass from substrate is 0.05 g g-1 . The maintenance coefficient is 2.0 g g-1 h -1 . The specific rate of product formation due to maintenance is 1.0 h-1 . The maximum specific growth rate of the organism is 0.3 h -1 . YPX= 7.7 and product formation is directly linked with metabolism. a) What is the time required to produce 15 g of biomass? !!!!! ANS IS 3.07 HRS!!!! STEPS on how to reach this pleaseMention two current commercial and environmental applications of Penicillium sp. Predict the microbe's probable applications.Why would farmers be concerned about nitrifying bacteria?