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Determine whether the following statement regarding eukaryotic
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- Briefly discuss the pros and cons of having a nucleoid (as bacteria do) versus a double nuclear membrane surrounding the DNA (as in eukaryotes). List and explain three reasons why DNA replication is very accurate.If the sequence of one single strand of DNA is C-A-A-G-T-A-G-G-C-T, what is the sequence of the complementary strand? Describe the origin of each strand of the new double helices created after DNA replication. Why is DNA replication important to the growth and development of a multicellular organism? Place the following terms in the correct order from smallest to largest: Nucleosome, supercoils, coils, chromosome, DNA double helixBelow is a diagram of DNA replication as currently believed to occur in E. coli. Arrows start from numbers and end at specific points. Answer the questions relating to the locations specified by the numbers (1) Which end (5' or 3') of the molecule is here? (2) Which enzyme is probably functioning here to deal with supercoils in the DNA? (3) Which enzyme is probably functioning here to unwind the DNA?
- Explain what semiconservative means with respect to DNA replication.Why is DNA replication is considered a semi-discontinuous process? Explain in detail.For the following steps in DNA replication, name the prokaryotic and eukaryotic enzymes (in that order!) that perform the task listed: Question 33 options: Tethering a polymerase to DNA (prokaryotes) Removing supercoils (eukaryotes) Removing supercoils (prokaryotes) Lagging strand replication (eukaryotes) Lagging strand replication (prokaryotes) Tethering a polymerase to DNA (eukaryotes) Stabilizing single-stranded DNA (prokaryotes) Stabilizing single-stranded DNA (eukaryotes) 1. DNA Pol III 2. DNA Pol Delta () 3. DNA Pol Epsilon () 4. Helicase 5. Topoisomerase (type I or II) 6. Beta clamp 7. PCNA 8. SSB 9. RPA 10. DNA Pol I 11. Ligase
- Considering prokaryotes, what term adds nucleotides in the 5' to 3' direction during DNA replication?Below is a diagram of DNA replication as currently believed to occur in E. coli. From specific points, arrows are provided that lead to numbers. Answer the questions below relating to the locations specified by the numbers. (02) What end (5’ or 3’) of the molecule is here? (State which) What enzyme is probably functioning here to deal with supercoils in the DNA? What enzyme is probably functioning here to unwind the DNA? What nucleic acid is probably depicted here? What are these short DNA fragments usually called? What enzyme probably functions here to couple these two newly synthesized fragments of DNA? Is this strand the leading or lagging strand? What end (5’ or 3’) of the molecule is here? (State which)DNA Replication occurs on both prokaryotes and eukaryotes. Although they have a similar genetic flow, there are small differences in between. What are the differences of DNA replication in prokaryotes and eukaryotes? What is/are the major difference/s?
- Explain the complexities of DNA replication that make the process (a) bidirectional and (b) continuous in one strand and discontinuous in the other.Identify two important enzymes involved in replication. Where does replication occur in the cell? What is the product of replication? Why is replication important to the cell and living organism? What is the process of copying DNA called?With regard to DNA replication, define the term bidirectional replication.