Determine the RIGHT order of a standard molecular biology workflow for species identification and classification. 1 2 3 4 5 6 7 ⠀⠀⠀⠀ DNA extraction Primer design DNA quantification (AGE and UV-Vis Spectrophotometry) Genomic/DNA sequencing DNA sequence processing and analysis PCR DNA quantification (Confirmation of PCR product via AGE)
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- Discuss the uses of PCR and RT-PCR in human identity (e.g. paternity, forensics or criminal cases) as well as in viral or bacterial DNA/RNA analysis (i.e. Identification of SARS-COV-2 RNA coronavirus using RT-PCR).An integrated tool used for conducting automatic and manual sequence alignment of DNA and protein, and inferring phylogenetic relationships between identified sequences. NCBI: GenBank NCBI: Primer Blast Tool NCBI: Nucleotide Blast Tool Molecular Evolutionary Genetics Analysis (MEGA)1. Definitions and brief statements 1. DNA Data Bank of Japan 2. MeSH Database 3. Please describe the 3 steps and temperature that are cycled during a PCR reaction. 4. What are global alignment and local alignment and their algorithms? ( 5. ProtScale
- Select all that would be true if I had a missense mutation in an gene: The missense mutant protein would be the same size by Western as the wildtype protein The missense mutant allele would be a different size compared to wildtype by PCR- electrophoresis The missense mutant protein would be a different size by Western compared to the wildtype protein The missense mutant allele would be the same size as wildtype by PCR-electrophoresisOrder the following technical steps that are required to identify a bacterium by PCR and sequencing of the 16S rRNA gene. [1 mark] - Carry out thermal cycling (amplification) of the PCR - Checking the PCR product (amplication) by agarose gel electrophoresis - Purify the PCR product - Perform a DNA extraction - Sequence the PCR product(Sanger sequencing) - Grow a fresh overnigh liquid culture - Obtain a pure bacterial culture - Set up a 16S Rrna gene PCR - Perform bioinformatic analysis (BLAST)Regarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genome
- Answer the following: What is DNA fingerprinting? Name 2 applications of DNA fingerprinting from lecture. • Describe the steps of DNA fingerprinting. Include the following terms underlined: o DNA sample o PCR o restriction enzyme o gel electrophoresis If you haven't done so already, explain the steps of PCR in detail - also explain the steps of gel electrophoresis in detail.Which of the following is NOT an activity carried out in the field of bioinformatics? a. collecting and storing DNA sequence information produced by various genome sequencing projects b. analyzing genome sequences to determine the location of genes c. determining the three-dimensional structure of proteins d. comparing genomes of different species e. none of theseMolecular biologists rely on many, often sophisticated, techniques to pursue their discipline. One may list ultracentrifugation, electron microscopy, X-ray diffraction, electrophoresis, and computer interfacing as fundamental tools. Model organisms provide the raw materials for study. List three "organisms" (or organismic groups) often used by recombinant DNA technologists and describe a major advantage of each group.
- Explain how biotechnology and bioinformatics are used to identify microorganisms Explain why biotechnology has replaced many of the traditional diagnostic tools in microbiology Explain why a PCR reaction needs a source of nucleotides in the form of ATP, GTP, CTP and TTP. Explain what primers are and describe their use in PCR reactions Given the results from a PCR/Gel electrophoresis or a DNA sequence; determine the identity of a bacterium or virus...Compare and contrast selective breeding with rDNA technology. Describe a minimum of three (3) advantages to rDNA technology. (Relates to GMOS)Nucleic acid hybridization techniques have been essential tools of molecular diagnostics for plant, animal and human diseases. Describe how the following hybridization techniques are Nucleic Acid Hybridization Technique Northern Blot Western Blot Transblot Molecules involved (DNA, RNA or Protein) Steps involved Immobilization phase (Medium) Electrophoretic run (Yes or No) Confirmatory test for hybridization Detection method for hybridization Potential applications