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Describe problems associated with the use of viral
vectors to introduce therapeutic genes into the cells
of patients.
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- discuss the benefits and limitations of using a retroviral system or an adenovirus for the delivery of a therapeutic gene.. a. Compare the means by which retroviral and AAVvectors deliver therapeutic genes to human cells.b. Explain the advantages and disadvantages of eachof the two viral vectors.Explain the main defects of using animal models for viral growth and cultivation.
- Aside from antibody-producing vaccines against spike proteins, describe at least 2 other therapeutic strategies that employ knowledge of pathways allowing viral entry into cells and/or mechanisms preventing replication of new viral particles after entry.Knowing that retroviruses operate on the principle of reversing thedirection of transcription from RNA to DNA, propose a drug thatmight possibly interfere with their replication.Antiretroviral drugs target various aspects of HIV replication and infection. Which of the following enzymes are targets of antiretroviral drugs? Select ALL that applya).reverse transcriptaseb)integrasec).ligased).proteasee).DNA polymerase
- Explain how therapeutic genes may be delivered topatientsDiscuss some advantages and disadvantages of bacteriophage therapyin treating bacterial infections.Discuss the principles behind the use of the following techniques in the diagnosis of viral infections, providing examples of viruses diagnosed using these methods: Restriction Fragment Length Polymorphism
- Explain the purposes of boosters, adjuvants, Trojan horse vaccines,and toxoids.b) Explain how the chimpanzee adenovirus has been genetically modified and developed as a viral vector to deliver the Oxford-Astrra Zenica Covid 19 vaccine (ChAd0x1nCoV-19/AZD1222). Include how the vaccine was developed, trialled, how the vaccine works and why a booster is needed.explain Resistance plasmids