Describe how LINE 1 element transposition occurs.

The map shows a wild-type HOAP gene, whereas Mutant 1 has a DNA deletion from base pair 1125 to 1445 and Mutant 2 contains a point mutation at 1372. Predict the sizes of DNA fragments that would be generated in each of the following digests. Wild-type (BamHI): Wild-type (EcoRI): Wild-type (BamHI + EcoRI): Mutant 1 (BamHI): Mutant 1 (EcoRI): Mutant 1 (BamHI + EcoRI): Mutant 2 (BamHI): Mutant 2 (EcoRI): Mutant 2 (BamHI + EcoRI): BamHI O EcoRI 740 Promoter ECORI 1140 HOAP HOAP HP1/ORC Associated Protein 7200 bp Sacl 2100 BamHI 2420 Psti 2700 Hindlll 3440

An interrupted mating study is carried out on Hfr strains 1, 2, and 3. After conjugation is established, a small sample of the mixture is collected every minute for 20 minutes to determine the distance between the genes on the chromosome. Times of entry for each of the three Hfr strains studied in the interrupted mating are provided in parenthesis. The total duration of conjugation (in minutes) is given for each transferred gene. Hfr Strain Genes Transferred Hfr 1 oriT(0)  met(2)   ala(8)  lac(13)  gal(17) Hfr 2 oriT(0)  met(2) leu(7)  thr(10)  azi(17) Hfr 3 oriT (0)  gal(3)  pro(8)  trp(14)  azi(19) Hfr 4 oriT leu met ala lac Hfr 5 oriT trp azi thr leu a. Use the data to construct a circular map of the bacterium.

Which steps in the double-strand break model for recombinationwould be inhibited if the following proteins were missing? Explainthe function of each protein required for the step that is inhibited.A. RecBCDB. RecAC. RecGD. RuvABC