Flow cytometry data!!!! PEAKS!PLEASE help me to understand how to read the peaks values (1-8) from the flow cytometry data (have uploaded the file ''wildtype'').(My strain is wildtype) THANK YOU!!!!!! ResultsTable 3a Flow cytometry data:StrainWild typednaA46mutantMean Fluorescense (BV605) of peaks from sample 1 a5 g.e 6 g.e.7 g.e.peak peak peak1 g. 2g.e. 3 g. 4g.e.peak peak peak peak8 g.e.peakg.e, is a genome equivalent; for E. coli this is the amount of DNA in one circularchromosome. SSC-A29²150 200 250 300CountExperiment Name:Specimen Name:Tube Name:Record Date:SOP:GUID:Display RangePopulationP1AllP2(¯---------P4P5P7P8P9Hold M-M11 1aGlobal Sheet2FSC-A#Events10,0009.9576,4932,320778671771259.64810²P2BD FACSDiva 8.0.1.1100%Parent100.099.664.923.20.80.90.70.8m.mi1.396.5Hold M-M11 1aBV605-AHold M-M11 1a150BV605-AMin-151109SSC-A1931.9175,1338,0107,0367.4587,8258,151709M11_1aSep 16, 2022 12:47:23 PMuser110BV605-AMax749.160749,1603.9726,3798.9067,9068.2918,708P$9.7847,821Pe200Blank Experiment with Sample Tube_2020_E og M_...Hold Mf71b56df-b57b-49e8-acfb-25ec126911a9Linear Scale (0-10.000)F7P4SSC-AMean2,1732,0571,3841,6812,6243,0322,895P82,9272.6221,469250(1,000)BV605-AMean4,2614,2802.9175,7468,4547,4407,8598,2888.9063,758Printed on: Fri Sep 16, 2022 06:42:17 CEST

Answered: Count Experiment Name: Specimen Name:…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Flow cytometry data!!!! PEAKS!

PLEASE help me to understand how to read the peaks values (1-8) from the flow cytometry data (have uploaded the file ''wildtype'').

(My strain is wildtype)

THANK YOU!!!!!!

Results
Table 3a Flow cytometry data:
Strain
Wild type
dnaA46
mutant
Mean Fluorescense (BV605) of peaks from sample 1 a
5 g.e 6 g.e.
7 g.e.
peak peak peak
1 g. 2g.e. 3 g. 4g.e.
peak peak peak peak
8 g.e.
peak
g.e, is a genome equivalent; for E. coli this is the amount of DNA in one circular
chromosome.

SSC-A
29²
150 200 250 300
Count
Experiment Name:
Specimen Name:
Tube Name:
Record Date:
SOP:
GUID:
Display Range
Population
P1
All
P2
(¯---------
P4
P5
P7
P8
P9
Hold M-M11 1a
Global Sheet2
FSC-A
#Events
10,000
9.957
6,493
2,320
77
86
71
77
125
9.648
10²
P2
BD FACSDiva 8.0.1.1
100
%Parent
100.0
99.6
64.9
23.2
0.8
0.9
0.7
0.8
m.mi
1.3
96.5
Hold M-M11 1a
BV605-A
Hold M-M11 1a
150
BV605-A
Min
-151
109
SSC-A
193
1.917
5,133
8,010
7,036
7.458
7,825
8,151
709
M11_1a
Sep 16, 2022 12:47:23 PM
user1
10
BV605-A
Max
749.160
749,160
3.972
6,379
8.906
7,906
8.291
8,708
P$
9.784
7,821
Pe
200
Blank Experiment with Sample Tube_2020_E og M_...
Hold M
f71b56df-b57b-49e8-acfb-25ec126911a9
Linear Scale (0-10.000)
F7
P4
SSC-A
Mean
2,173
2,057
1,384
1,681
2,624
3,032
2,895
P8
2,927
2.622
1,469
250
(1,000)
BV605-A
Mean
4,261
4,280
2.917
5,746
8,454
7,440
7,859
8,288
8.906
3,758
Printed on: Fri Sep 16, 2022 06:42:17 CEST

Expert Solution

Step 1

The flow cytometry data in the given image uses BV-605 as the fluorescent molecule. From the results, it seems that the cell cycle analysis was done using this molecule as fluorochrome in which percentage of cells in different phases of cell cycle (Sub-G0/G1, G0/G1, S and G2M) can be determined. Sub-G0/G1 phase corresponds to dead cells, G0/G1 phase corresponds to cells in G1 phase preparing for DNA replication, S phase is for cells undergoing DNA replication and G2M phase corresponds to cells preparing for cell division.

Firstly, as performed in flow cytometry, FSC and SSC were plotted to gate the desired cell population. From this data, P1 cells were gated which can be seen as cluster of cells. Then, P1 gated cells were assessed for the BV605-A positive cells shown as P9 population. Following this, cells in different cell cycle phases were found.