Consider the spectrophotometric pH measurement of seawater with the indicator thymol blue illustrated at the opening of this chapter. The blue form of the indicator In22 on page 202 has maximum absorbance at a wavelength of 596 nm (nanometers). The yellow form HIn2 has maximum absorbance at 435 nm. Measurements of absorbance at the two wavelengths allows us to find the ratio [In22]/[HIn2]. How would you use this measurement to find the pH?

General, Organic, and Biological Chemistry
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Chapter10: Acids, Bases, And Salts
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Consider the spectrophotometric pH measurement of seawater with the indicator thymol blue illustrated at the opening of this chapter. The blue form of the indicator In22 on page 202 has maximum absorbance at a wavelength of 596 nm (nanometers). The yellow form HIn2 has maximum absorbance at 435 nm. Measurements of absorbance at the two wavelengths allows us to find the ratio [In22]/[HIn2]. How would you use this measurement to find the pH?

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