Consider the reaction catalyzed by PFK. In the presence of AMP, which of the following will be expected? Check all that apply: a)The Km for substrate would be decreased b) the initial velocity plot would show the curve shifted to the right c) the R state is stabilized d)the rate of the reaction is diminished

Q: For the reaction A ==> B, Delta G'° = -60 kJ/mol. The reaction is started with 10 mmol of A; no B is…

A: Step 1:Step 2: Step 3: Step 4:

Q: The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Q: kinetically perfect enzymes a) Using the Gibbs energy formula, explain why engergonic reactions can…

A: Most enzymes follow Michaelis -Menten (MM)kinetics and hence obeys the MM equilibrium assumption…

Q: For the following reaction: k1 E+S ES P+E (a) What is the rate equation for the formation of ES? (b)…

A: Enzymes are biological catalysts that help in catalyzing or speeding up biological reactions by…

Q: Hemoglobin is the oxygen carrier in the blood. What is the effect of each of the following…

A: Hemoglobin is considered a globulin protein in the red blood cells, It plays a significant role in…

Q: A biochemical reaction transfers 60 kJ mol-1(15 kcal mol-1) of energy. What general process most…

A: Biochemical Reaction : It is the transformation of one molecule to a different molecule inside a…

Q: Which of the following is not true for the nicotinamide cofactors NAD and NADP: O A) The oxidized…

A: FEEL FREE TO ASK FOR CLARIFICATIONS

Q: Please balance the reaction below. Considering the pe0 of Pb2+/Pb0 and O2/H2O are -2.13 and 14.5,…

A: Pb0/Pb2+ will occur at anode as this is oxidation reaction as Pb gets oxidized  O2/H2O will pccur at…

Q: You have discovered a new enzyme that has a nearly identical active site to chymotrypsin. This new…

A: As given peptide is Asn-Phe-lys, and discovered enzyme cleaves at C terminal of non ionizable polar…

Q: Calculate the value of AG, if the value of AH is +1200 Kcal/mol, AS is +60 Kcal/mol, and the…

A: To find the Gibbs free energy change (ΔG) using the given values, we use the Gibbs free energy…

Q: The table below provides kinetic information when ADH is reacted with ethanol alone, NAD+ alone, and…

A: Enzyme kinetics is the study of an enzyme catalyzed biochemical reactions. Usually it is studied by…

Q: Why is the overall coupled reaction exergonic?

A: The coupled reaction like a redox reaction which is coupled or combined.

Q: Sketch on one reaction rate vs. substrate concentration graph & sketch on one Lineweaver-Burk type…

A: LB plot is double reciprocal plot which gives Km and Vmax values as x intercept and y intercept…

Q: An enzymatic reaction was carried out in a batch-operated reactor until exhaustion of the substrate.…

A: Michaelis-Menten equation is used for analyzing the rate of the enzyme-catalyzed reactions in a…

Q: Cooperativity yields a sigmoidal kinetic curve. Which of the following is TRUE?

A: Co-operativity can be described as a phenomenon where the enzyme shape changes with the substrate…

Q: An increase in PCO2 will result in which of the following? O a) An increased affinity between PO,…

A: Haemoglobin oxygen dissociation curve is a sigmoidal curve. A shift of the curve to the right…

Q: Most of the enzyme reactions followed the mathematical kinetic plots suggested by Michaelis-Menten…

A: Michaelis-Menten plot is a construct that helps to determine the enzyme activity using varying…

Q: To purify a given enzyme from a crude extract that contains it, fractional precipitations, exchange…

A: Protein purification is necessary to purify the protein to homogeneity. Homogeneous protein sample…

Q: For the following two scenarios, sketch the complete reaction free energy diagram for an…

A: The enzymes are involved in increasing the reaction rate of a biochemical reaction. A reaction is…

Q: Assume an enzyme has a loss-of-function mutation that completely abolishes the activity of that…

A: Alleles with wild type alleles usually code for a product that is required for a certain biological…

Q: Predict the major product of each of the following reactions or series of reactions. In cases…

A: a) In the presence of FeBr3, the benzene ring that has a carbonyl group (a ketone) attached is…

Q: With reference to your experimental protocol, why does adding the reaction mix to the “stop” tubes…

A: Alkaline phosphatase (ALP) is an enzyme that catalyzes the hydrolysis of phosphate esters in an…

Q: With the help of the half reactions given in Table 1, formulate the redox equation for the oxidation…

A: FADH2 skips Complex I of the electron transport chain and donate the electron it is carrying to…

Q: How can we test the validity of the mechanism proposed for the catalytic triad?

A: A set of the 3 coordinated amino acids that can be present on the active site of some of the enzymes…

Q: To characterize a purified enzyme, a biochemist has determined the reaction initial velocities, at…

A: Enzymes are usually protein catalyst which catalyzes several biochemical reactions. Inhibitors (I)…

Q: graph shows the reaction coordinate of an enzymatic reaction of substrate to product a) which number…

A: When substrate converts into product Activation energy is required. Activation energy is the minimum…

Q: Given the following reaction and equation for the initial velocity of the reaction: k₁ k3 E+SES E +…

A: We are given two equations for initial velocity (V). V=kcat [ES]    -------(eq1)       andV=k3 [ES]…

Q: You have discovered a new enzyme that has a nearly identical active site to chymotrypsin. This new…

A: It is mentioned that the newly discovered enzyme with a reaction mechanism similar to that of the…

Q: 31) Four catalytic strategies include all but the following: A) Noncovalent catalysis B) Covalent…

A: Enzymes are biocatalysts that speeds up the reaction process. They are generally proteins and they…

Q: Consider the following reaction at 298K. Fe2+ (aq) + Sn (s) — Fe (s) + Sn²+ (aq) Which of the…

A: To determine which statements are correct, we need to analyze the reaction:…

Q: An enzyme with an unknown reaction mechanism has been isolated and is being investigated in the lab.…

A: General acid/base: proton transfer occurs between enzyme and substrate. Note that water is not a…

Q: Derive an Equation that explains the realtionship between kE and kN with respect to the equilibrium…

A: Equation: This reaction is governed by the equilibrium constant ​, where:       - (1)Assuming that…

Q: For some Enzyme, the Vmax is 18 micromols/min, Km is 400 microM. If the substrate concentration is…

A: Velocity of the reaction defines the rate of reaction which is catalyzed by the enzyme. It depends…

Q: Under standard conditions, will the following reaction proceed spontaneously as written?

A: A chemical process in which one substance changes into another is known as a chemical reaction. A…

Q: How to identify the Vmax and the Michaelis constant (Km) in an enzyme kinetics graph?

A: An enzyme with a high Km requires a higher substrate concentration to achieve a given reaction…

Q: Which of the following is TRUE under the following conditions: the enzyme concentration is 2.5 nM,…

A: The rate of reaction can be determined by using Michaelis Menten equation. Michaelis Menten equation…

Q: An uncatalyzed reaction has a rate of 4.2 x 10-7 sec-1. When an enzyme is added the rate is 3.2 x…

A: Enzymes are proteins present inside organisms, which function to work as biological catalysts. These…

Q: You begin to study enzyme Z, which catalyzes a simple reversible reaction that interconverts…

A: S to P conversion is said to have a  of -6 kJ/mol. So, let's give 'S' a free energy (G) value of 0…

Q: For an enzyme with a substrate Km of 0.001 M. a) What substrate concentration for an initial rate…

A: Given Values: Km = 0.001 M

Q: Consider the mechanism below. (i) Identify Asp X's function in the first step of the reaction. Ex…

A: Amino acids in the active site of enzymes can acts as general acids and/or general base. Amino acids…

Q: Consider the analogy of the jiggling box containing coins that was described on page 85. The…

A: When we face a situation where rate of forward reaction is euqal to the rate of backward reaction…

Q: The amine 1 is an anti-cancer drug that is made using the following reaction, which is catalysed by…

A: NADPH is the most favored reducing equivalent used by living organisms in various metabolic…

Q: In a alkaline phosphatase kinetics experiments a studnet first made a standard curve with different…

A: Alkaline phosphatase enzyme catalyzes PNPP (substrate) into PNP (product). Enzyme kinetics is the…