Consider the culture type in which growth was distributed throughout the entire medium and explain why the growth was more abundant toward the surface of the medium in some cultures, whereas other cultures showed an equal distri- 5. bution of growth throughout the tubes.

Determine what percentage of the culture was living (viable) and what percentage was dead (mortality).  Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count  Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6

If 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of the original culture

Give two reasons for heating the slide after the smear is air dried?

Below is a diagram of the serial dilution of a culture with 1 x 10º cells. Fill out the missing information. 250 Culture Diluent 1 x 10° cells/mL 9 mL 9 mL 9.9 mL 9.9 mL 99.9 mL Volume to add to diluent Final Dilution level Theoretical count after plating 100 uL

Examine figure a, b (shown here). If you performed the quadrantstreak plate method using a broth culture that had 10× fewer cellsthan the broth used in the culture shown, which quadrant might youexpect to yield isolated colonies? Explain your answer.

Answer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity?  When can gelatin be used in culture media preparation?  How does cotton plug prevent contamination of the medium?  Why should caps of glassware be loosened first before sterilization?

How could calculate this serial dilutions probelms for microbiology and how do can I know what tube has "10^(_)" when making dilutions?

Derailed description of the following test / media  SIM medium

the image contains results of the fluid thioglycolate medium tests. Please explain the results and the chemistry of each tube? Thank you