Consider the culture type in which growth was distributed throughout the entire medium and explain why the growth was more abundant toward the surface of the medium in some cultures, whereas other cultures showed an equal distri- 5. bution of growth throughout the tubes.

Q: Antibiotics should be reduced or eliminated from culture media during cell subculturing. Make it…

A: Subculturing, also known as passaging cells, is the process of removing the media from an old…

Q: Determine what percentage of the culture was living (viable) and what percentage was dead…

A: The word "CFU" in microbiology refers to a colony-forming unit, which is used to estimate how many…

Q: What medium is this? What is it detecting? Why do you see the clear ring around one bacteria (how…

A: Caesin Hydrolysis Test This test is used to determine the ability of the provided bacteria to…

Q: Using one of the graphs obtained with the medium II, name the different phases of the bacterial…

A: Micro organisms are typically grown in a closed culture media. Nutrients are added according to the…

Q: What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for?…

A: Gelatin, also known as gelatine is a translucent, tasteless flavorless component that is often made…

Q: This is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to…

A: There are 5 phases to a logistic (sigmoidal) growth curve.  They are stationary phase, lag phase,…

Q: Is the Tryptoc Soy Agar (TSA) a complex defined medium? Explaining based on its composition?  Is TSA…

A: Trypticase soy agar  (TSA) and Trypticase soy broth is a growth media for the culturing of bacteria.

Q: Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution…

A: Serial dilutions is a process of making step by step dilutions of a substance. It is formed by…

Q: A bioreactor which is used for the production of growth factor A is operated as fed- batch culture.…

A: A Bioreactor is a device or a process system which supports and habitats the biologically active…

Q: Some exconjugants did not grow on minimal medium. On what medium would they grow?

A: Exconjugants can be described as bacterial cells, which separates following the conjugation. A…

Q: Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial…

A: Organism / MediaNutrient Agar (NA)Eosin Methylene Blue (EMB)Mannitol Salt Agar (MSA)Escherichia…

Q: a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total…

A: Since, the question a. in incomplete as it requires figure 2.9 to be answered we will answer the…

Q: Write instruction to make cell culture medium using DMEM consist of 10% FBS, 1% streptomycin.

A: Introduction Streptomycin is an antimycobacterial drug which is used in the treatment of…

Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar…

A: Aseptic Technique describe some of the ways that a laboratory can deal with the constant threat of…

Q: With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable…

A: Bacteria are the prokaryotic, unicellular organisms that are sometimes pathogenic in nature. The…

Q: The cell concentration measured in samples taken at certain intervals from a microorganism growth…

A: From the given table,   Cf, final cell concentration =0.376  Ci, initial cell concentration=0.01…

Q: Your colleague has given you an unauthenticated cell culture grown from a primary explant. Suggest…

A: Mycoplasma contamination of cell cultures is extremely common as these bacteria are found commonly…

Q: Calculate the growth rate and generation time of a culture that increases in the exponential phase…

A: The growth of bacteria over a period of time is represented by a growth curve. This curve tells the…

Q: The factors for the manipulation of culture medium?

A: Culture medium It is also called a growth medium. It is a solid, liquid, or semi-solid medium that…

Q: Based on these results, which of the following statements is true regarding the effectiveness of…

A: A disinfectant is a chemical substance or compound that is used to inactivate or destroy the…

Q: Describe the appearance of an S. agalactiae colony grown on blood agar. Describe how that colony…

A: The initial identification of the enterococcal and streptococcal species is based on their hemolytic…

Q: After streaking microbial culture on agar plates and observing colonial growth, TMTC usually…

A: TMTC ("Too Many to Count") is a condition in which the number of colonies on an agar plate exceeds…

Q: How could calculate this serial dilutions probelms for microbiology and how do can I know what tube…

A: Serial dilution is a method of step-wise dilution of bacterial culture to reduce the concentration…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: e the process to make a 4-phase streak plate beginning with your first streak (i.e you have…

A: The sample/inoculum is usually pored by streak with the diluted through streaking it across the…

Q: Calculate the colony forming units (CFU) if 100  colonies grow in agar using  a 0.01-mL calibrated…

A: One colony equals to 100 col/mL in  0.01 -mL calibrated loop.

Q: A tampered sack of powdered milk was analyzed by getting 50 grams of composited sample and diluting…

A: Dilution : We know that the solute of the concentration will be decreasing in solution . There is a…

Q: What is the effect of trypsin EDTA solution on monolayer cell culture preparation?

A: Trypsin is an enzyme which helps in digestion of proteins.In the small intestine,proteins are broken…

Q: Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the…

A: SpectrophotometryIt refers to a quantitative measurement of how different substances absorb or…

Q: What is the advantage of the plating method over an electronic cell counting method in counting…

A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…

Q: If an organism that showed no growth in the MIC broth test at a particular dose, then grows in an…

A: Microbes are present everywhere and the different types of microbes are present. Microbes that cause…

Q: When viable cell concentrations are too high to count on an agar plate, it is common to use larger…

A: The correct option is shown below.

Q: laboratory? explain with diagram

A: The tissue culture is the general term for the removal of the cells, tissues or organs from an…

Q: Y ΝΑ XIW Hfr NA Nutrient Agar Provides all nutrients the bacteria needs + no antibiotics y ECM XIW…

A: The ECM agar is composed of the minimum nutrients required for the growth of bacterial cells,…

Q: Define the following termonologies in brief :  Focal Adhesion Biofilm  Calcification and examples…

A: "Since you have asked multiple parts of the same question, we are eligible to answer only the first…

Q: A culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be…

A: Dilution is the process of reducing the concentration of a solute in a solution. This is typically…

Q: During the heat shock step, a student accidentally heated the cells at 42°C for 5 minutes. Only a…

A: The heat shock step is an important part of certain molecular biology techniques, such as…

Q: 1a)A surface sterilisation protocol was carried out to sterilise leaf and stem explants for…

A: Surface sterilization is the process of removal of contaminants present on the surface of leaves and…

Q: A student plates 100 µL of a 107 dilution of E. coli culture on a Luria Agar plate. After 24 hour,…

A: The bacterial sample when taken from the stock solution, it may contain lots of bacterial cells, and…

Q: use these OD numbers to plot growth curve for E. coli K12– and estimate generation time for this…

A: The growth curve for E.coli K12 is plotted as follows:

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."