Colour of band Plant pigment Table 1. Chromatographic analysis of green leaf Distance moved by component Distance moved by Rf value (cm) solvent (cm) Light brown Chlorophyll B 1.5 8.9 0.17 Yellowish green Dark brown Chlorophyll B tannin 6.5 8.9 0.73 7.1 8.9 0.80 Table 2. Chromatographic analysis of the variegated leaf sample Colour of band Plant pigment Distance moved by component Distance moved by Rf value (cm) solvent (cm) Yellowish green Chlorophyll B 2.5 10.2 0.25 Light orange Carotene 5.5 10.2 0.54 Light purple Anthocyanin 7.2 10.2 0.71 Brown Tannin 7.5 10.3 0.74 Table 3. Chromatographic analysis for hibiscus sample Colour of band Plant pigment Distance moved by Distance Rf value component moved by (cm) solvent (cm) Pale yellow Pink Purple | Xanthophyll 1 9.5 0.1 Anthocyanin 5.5 9.5 0.58 tannin 6.9 9.5 0.73

Write how can prepare 5% gel for gel electrophoresis.

What is safe view dye for? Compare it with Ethidium Bromide.

Define the following terms:a.Simple stainingb.Complex mediumc.Defined (synthetic) mediumd.Parfocale.Negative stainingf. Resolving powerg.Magnifying power

discuss the method ATP-TOX as an old method and PCR as a new method for water biomentoring method and where does it fit in with existing testing and why is it relevent and new?

What is the difference between total magnification and objective magnification? You are looking at an organism on the 20x objective What is the total magnification? If the organism takes up 62 units on the ocular micrometer, how big is the organism is micrometers? Show your work.

Differentiate the “hot method” and “cold method” of acid-fast staining

What are the advantages and disadvantages of using the Wet Mount technique? What are the advantages and disadvantages of using the Hanging drop technique? What are the advantages and disadvantages of using the Slide Culture technique? pls elaborate each, thank you

What is the principle of the gel electrophoresis separation technique?

3d bioprinting systems, biomaterials, tissue engineering.Find a creative project topic on the above-mentioned area.