This test determines the ability of bacteria to use citrate as source of carbon and inorganic ammonium salt as a source of nitrogen. The following are the stepwise procedure in conducting citrate test: 1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needle to a colony. 2. Incubate at 37 degree Celsius for 24 to 48 hours.   Task: In a tabulated form, determine if the Escherichia coli are positive or negative with citrate test. Interpret your answer and include the reaction of organism on this test.

Which of the following tests will help me differentiate between an unknown bacteria that is either Salmonella typhimurium and Serratia marcescens Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Catalase Test

Explain the following tests using Pseudomonas aeruginosa.  Describe the purpose, reagents used and the results using Pseudomonas aeruginosa.

How can I explain the results of my findings in lamen terms? 1 - Name of Test Type of Medium 2 - Color Before Incubation 3-Color After Incubation? 4 – Results Positive/ Negative? 5 - Reagents Added Lactose Fermentation test Phenol Red Lactose (PRL) Red Color: Yellow   Gas Bubble: Yes Positive Phenol Red to detect color change (added) Glucose Fermentation Test Phenol Red Glucose (PRG) Red Color: Yellow   Gas Bubble: Yes Positive Phenol Red to detect color change (added) Citrate Utilization Simmons Citrate Green Color: Blue Positive Bromthymol Blue (added) Indole Test Media = SIM Clear Indole: Color: Clear Negative For Indole, we add Kovac’s   Hydrogen Sulfide Test Media = SIM clear Hydrogen Sulfide: Color: Clear Negative Iron already added Methyl Red Test (MR test) Media = MRVP Broth Clear Color: Yellow Negative We add Methyl Red Reagent Voges-Proskauer Test (VP test) Media = MRVP Broth Clear…