This test determines the ability of bacteria to use citrate as source of carbon and inorganic ammonium salt as a source of nitrogen. The following are the stepwise procedure in conducting citrate test: 1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needle to a colony. 2. Incubate at 37 degree Celsius for 24 to 48 hours.   Task: In a tabulated form, determine if the Escherichia coli are positive or negative with citrate test. Interpret your answer and include the reaction of organism on this test.

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.