Cite the qualitative tests used to characterise the RNA hydrolysis products. Indicate the chemicals used in the procedure and the good outcome: The test of A. Bial Test for B.Murexide

Q: The following set of data are measurements of zones of inhibition (in mm) from a Kirby-Bauer test.…

A: The Kirby-Bauer test, also known as the disc diffusion test, is a standard method used to determine…

Q: Provide details about the reation workup. How is this product purified , and what methods were used…

A: The objective of the question is to understand the process of reaction workup, purification, and…

Q: Provide the different qualitative and quantitative tests for amino acids in this format:

A: Amino acid is the basic unit of a protein. Amino acids contain an amine group, a carboxylic acid…

Q: What is the color results of tyrosine,phenylalanine,alanine in millon test?

A: Million's test is a protein qualitative test: Principle: Million's test detect tyrosine as its the…

Q: Match the test with the appropriate reagent/ chemical  a. DNA test b. Paper chromatography  c.…

A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…

Q: What methods did the authors use to prove they have the right material?   4,4'-DIBROMOBIPHENYL…

A: The objective of the question is to understand the methods used by the authors to confirm that they…

Q: Repeated freezing and thawing, sonication, French press, etc. are some of the methods for enzyme…

A: These steps are performed for cell disruption to release the desired biomolecules from cells. This…

Q: Give a short explanation of the process of the DNS (dinitrosalicylic acid) assay/method in…

A: Estimation of Reducing sugar by DNS (Dinitrosalicylic Acid). Principle:       The reducing sugar…

Q: if glycogen is subjected directly to nelson's assay without prior hydrolysis

A: Nelson's assay method or Nelson-Somogyi method is used for the quantitative determination of…

Q: List some conditions that cause false positive reactions with the RPR test?

A: Syphilis, a sexually transmitted infection brought on by the bacteria Treponema pallidum, is…

Q: Match the biochemical test mediums to the chemical components for test reaction: OTSI TSI and SIM…

A: Biochemical test media are specialised growth mediums created to promote the growth and metabolic…

Q: Between Proteus mirabilis and Proteus vulgaris which are positive or negative for ornithine…

A: ornithine decarboxylase test is a special kind of biochemical test that helps to determine whether…

Q: If you had cultures of B. subtilis and E. aerogenes and forgot to label them, which of the following…

A: Some bacteria produce indole by deamination of amino acid tryptophan and shows positive indole test…

Q: Which of the following is similar to a block of highly purified “jello-like” substance that is not…

A: Micropippete is laboratory equipment used to transfer small quantities of liquid. Restriction…

Q: Can anyone assist in providing a detailed description of each step of the following purification…

A: Pectinase are group of enzymes which catalyzes the degradation of pectic substances. It occurs…

Q: This test gives yellow solution, indicating the presence of proline*   A. Biuret B. Ninhydrin C.…

A: Different of the tests mentioned here are tests for detection of amino acids. In the next step we…

Q: In detection of urea in urine what are the mechanisms, reactions, possible result and interpretation…

A: Urea is considered to be a waste product synthesized in the body by the oxidation of amino acids…

Q: Explain the effects of ampicillin and X-gal in the following 4 different LB media plates   LB LB +…

A: Ampicillin provides a means of selecting transformants that have taken up plasmid DNA containing the…

Q: A plant isolate was subjected to qualitative tests to determine the presence of macromolecules.…

A: Qualitative tests detect the presence of  specific molecules in a sample solution. There are…

Q: Differentiate the Guanidium-thiocyanate phenol-chloroform method from the silica membrane based…

A: Nucleic acids are involved in various processes in the cell, their main role is the expression of…

Q: An Enteropluri test (including VP), as used in Practical 4, gave the following results code: 7 2 3 O…

A: Digestive enzymes are categorized primarily based totally on their goal substrates: Lipases break…

Q: f four identical test tubes contains same amount of glucose, lactose, starch,  and sucrose solution.…

A: Carbohydrates are polyhydroxy aldehydes or ketones that are associated with reducing properties and…

Q: Describe two methods to test for the enzyme urease.

A: Urease is an enzyme that catalyzes the hydrolysis of urea forming ammonia and carbon dioxide. They…

Q: Chemical analysis of a patient’s urine using a reagent strip gave negative protein and negative…

A: Urinalysis is a diagnostic test performed over the urine sample collected from the patients. Results…

Q: Mention assays other than biuret and lowry assays that are used to detect the presence of proteins.…

A: Introduction: Protein is the major macromolecule that plays a vital role in the structure and…

Q: How do you determine protein concentration using bicinchoninic acid assay?

A: Proteins are polymers that are composed of amino acids. Amino acids are in turn made up of a central…

Q: You are given a complex, biological mixture and asked to determine the amount of penicillin present…

A: Antibiotics are compounds that either kill the bacteria (bactericidal) or control their growth…

Q: How do nucleotide analogs function as therapeutic agents? Explain the mechanism with specific…

A: FUNCTION 1. Nucleotide analogs are nucleotides and which contains a nucleic acid analogs,sugar and a…

Q: Think of another type of test to determine whrther a person is lactase persistent or nonpersistent.…

A: Lactase is an enzyme that is generated by a variety of species. It is found in the brush boundary of…

Q: Outline the steps to purify a protein. What criteria are used to evaluate purity?

A: Proteins are the polymers of amino acids residues which are arranged as a polypeptide chain…

Q: What are the clinical importance of the catalase and oxidase tests?

A: Two frequently used clinical microbiology diagnostic tests that aid in identifying bacteria based on…

Q: Briefly describe specifically how the presence of our enzyme of interest is being detected/observed…

A: The Urease test is a biochemical test used to detect the presence of the enzyme urease in…

Q: If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: What is the interpretation of adding the following during protein synthesis. 1) adding radioactive…

A: Radioactive substances have unstable atomic nuclei so they need to achieve a stable configuration by…

Q: Describe the tests used to identify Strep, and use a dichotomous key to successfully identify an…

A: Streptococcus is a gram positive bacteria. They are belong to the family Streptococcaceae.  They…

Q: Give at least two (2) chemical tests for amino acids/ proteins other than what were performed in…

A: In biochemistry , a chemical test is performed in qualitative or quantitative procedure to identify…

Q: Besides color, what indicates whether or not your bacteria have metabolized amino acids simply by…

A: In a KIA (Kligler Iron Agar) test, a yellow color in the medium indicates that the bacteria have…

Q: Describe the evidence of Picric acid test to show a positive result for the presence of a specific…

A: The picric acid test is one of the assays for identifying decreasing sugars. To find reducing…

Q: Explain the basis for identification using biochemical testing. – Again, discuss enzymatic pathways,…

A: Biochemical test is the conventional and common method which helps to identify microbes, generally…

Q: Provide examples of amino acids that would yield a positive Azo Dye test and amino acids that would…

A: The azo dye test is used to identify the presence of amino acids that contain the aromatic ring.…

Q: 1. Deduce the primary structure of this polypeptide. 2. Why would cyanogen bromide not be a good…

A: a) Given the molecular weight of the peptide is 4000Average MW of an amino acid is 110 Da. So, the…

Q: write a detailed description of each of the following tests/media: a. TSI Agar Slant b. SIM Medium…

A: The biochemical and microbial tests are performed to analyze the capability and function of the…