Choose two from the following culture preservations that are best to use when only an ordinary refrigerator is available for storage: a) Drying b) Mineral oil overlay c) Freezing with liquid nitrogen d) Lyophilization e) Periodic transfer to fresh medium
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Choose two from the following culture preservations that are best to use when only an ordinary refrigerator is available for storage:
a) Drying
b) Mineral oil overlay
c) Freezing with liquid nitrogen
d) Lyophilization
e) Periodic transfer to fresh medium
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- The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization?3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distille1. The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization? 3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilledTransparent double-sided dishes used for growing microbes are most commonly called A) Petri dishes. B) baker dishes. C) sterilization plates. D) culture medium plates.
- Listen Which of the following methods of microbial control is used most often with surgical equipment? a) autoclave b) sugar and salting c) dessication d) lyophilizationWhich of the following is used in the sterilization of the medium?a) benmargeb) swabc) autoclaved) centrifugee) coolingHow do you observe growth in liquid microbiological media? a) the medium is cloudy or turbid b) colonies appear on the surface of the medium c) the medium becomes solid d) colonies appear distributes throughout the medium
- Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri plate. B) a series of practices to avoid contamination. C) the autoclave and other sterilizing procedures. D) cleanliness in the laboratory.In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperBased on the knowledge acquired about the conservation and propagation of microorganisms, propose methodologies for:a) conservation of stock culture (used infrequently) of baker's yeast (S. cerevisiae);b) conservation of the working culture (used daily) of the baker's yeast;c) activation (1st stage of propagation) of the work culture;d) propagation of cultures until inoculating a 100 L reactor toi) production of baker's yeast;ii) ethanol production.
- Listen The following is a recipe for a bacterial growth medium: 35 g Dextrose, 12 g Agar, 1.2g Salt, 3 g Yeast cell extract, 20g tryptone. Based on the recipe, what type of growth medium would this be? A) Chemically defined growth medium B) Minimal growth medium C) Complex growth medium D) Chemically undefined growth medium E) More than one of the answers is correct.The use of sterile techniques and agar media enables microbiologists to study: a) Bacterial growth b) Nutrient agar-metabolizing culture c) Bacterial metabolism d) Pure cultures d) Bacterial life cyclWhy is dilution a necessary part of pure culture preparation?