Why do we use secondary antibodies in Western Blotting? Intensify signal from 1° antibody O Bind directly to an antigen O No answer text provided. Remove unbound 1° antibody

Which of the following assays would utilize monoclonal antibodies? MRI imaging of mice expressing viral constructs that express bioluminescence euthanization of mice infected with engineered viruses that express reporter proteins and staining for protein expression euthanization of SCID mice followed by dissection and flow cytometry euthanization followed by dissection and assaying for the virus in tissues

It’s not generation of junction of diversity

Using the diagram below answer the following: What is a role of minor H antigens in skin graft rejection process?  What is a difference between cases of graft rejected rapidly vs graft rejected slowly?  What requirements have to be met to insure that graft will be tolerated?  What testing should be performed to prevent rejection?

DTT and 2-ME would destroy which of the following antibodies? Group of answer choices Anti-Duffy Anti-E Anti-C Anti-Lea

Discuss the differences between conventional T cell therapy and Chimeric Antigen Receptor (CAR) T cells.

In immunofluorescence experiments, what is the role of the primary and secondary antibodies? (select all that applies) Group of answer choices The primary antibody binds the protein of interest whereas the secondary antibody binds the primary The primary and secondary antibodies can be interchangeable The secondary antibody is labeled with a fluorophore and is therefore responsible for generating the signal The secondary antibody is less important and therefore the experiment can be performed without it

1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge  true/false 3)  PBST  is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme  A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8

Maternal antibodies passed across the placenta both are IgM antibodies and are an example of natural passive immunity   are IgM antibodies   are an example of natural passive immunity   are an example of natural active immunity   both are IgM antibodies and are an example of natural active immunity

Why we use antibodies?  Please answer at your own words.