Enzymes BamHI EcoRI HindIII BamHI + EcoRI BamHI + HindIII EcoRI + HindIII BamHI + EcoRI + HindIII Fragment Sizes (in kb) 7.3, 3.2 10.5 5.1, 3.4, 2.0 6.7, 3.2, 0.6 4.6, 2.7, 2.0, 0.7, 0.5 4.0, 3.4, 2.0, 1.1 4.0, 2.7, 2.0, 0.7, 0.6, 0.5 Draw a restriction map for the plasmid that fits your data.

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14.25 You are studying a circular plasmid DNA molecule of
size 10.5 kilobase pairs (kb). When you digest this plas-
mid with restriction endonucleases BamHI, EcoRI, and
HindIII, singly and in all possible combinations, you
obtain linear restriction fragments of the following sizes:
Enzymes
BamHI
EcoRI
HindIII
BamHI + EcoRI
BamHI + HindIII
EcoRI + HindIII
BamHI + EcoRI + HindIII
Fragment Sizes (in kb)
7.3, 3.2
10.5
5.1, 3.4, 2.0
6.7, 3.2, 0.6
4.6, 2.7, 2.0, 0.7, 0.5
4.0, 3.4, 2.0, 1.1
4.0, 2.7, 2.0, 0.7, 0.6, 0.5
Draw a restriction map for the plasmid that fits your
data.
Transcribed Image Text:14.25 You are studying a circular plasmid DNA molecule of size 10.5 kilobase pairs (kb). When you digest this plas- mid with restriction endonucleases BamHI, EcoRI, and HindIII, singly and in all possible combinations, you obtain linear restriction fragments of the following sizes: Enzymes BamHI EcoRI HindIII BamHI + EcoRI BamHI + HindIII EcoRI + HindIII BamHI + EcoRI + HindIII Fragment Sizes (in kb) 7.3, 3.2 10.5 5.1, 3.4, 2.0 6.7, 3.2, 0.6 4.6, 2.7, 2.0, 0.7, 0.5 4.0, 3.4, 2.0, 1.1 4.0, 2.7, 2.0, 0.7, 0.6, 0.5 Draw a restriction map for the plasmid that fits your data.
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