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Benzoic Acid
V end point = 22 ml of NaOH
V 1/2 = 11 ml of NaOH
pKa = 5
Substituted Acid - P- bromobenzoic Acid
V end point = 17 ml of NaOH
V 1/2 = 8.5 ml of NaOH
pKa = 4.9
Briefly explain the electronic effect of the subsistent present in your acid compared to benzoic acid
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- A solution is prepared by diluting 2.79 mL of the blue dye stock solution to 25.00 mL. The measured absorbance for the prepared solution is: Blue dye stock solution = 0.293 M Absorbance at 630 nm = 0.00265 Calibration curve y = 0.0833x A.) What is the theoretical molar concentration? B.) What is the experimental molar concentration? C.) What is the percent error?An unknown amount of a compound with a molecular mass of 270.57 g/mol is dissolved in a 10 mL volumetric flask. A 1.00 mL aliquot of this solution is transferred to a 25 mL volumetric flask, and enough water is added to dilute to the mark. The absorbance of this diluted solution at 355 nm is 0.495 in a 1.000 cm cuvette. The molar absorptivity for this compound at 355 nm is €355 = 6149 M-¹cm-¹. What is the concentration of the compound in the cuvette? concentration: What is the concentration of the compound in the 10 ml flask? concentration: How many milligrams of compound were used to make the 10 mL solution? mass: M M mg1. at what wavelength was Pb determined? 2. What is the slope of the calibration curve? 3. What is the y-intercept of the calibration curve? 4. The researcher determined the Pb content of water sample from a river suspected to be contaminated with Pb from a nearby factory by complexing with cyanidine and the absorbance measured was 0.405. What is the lead content of the water sample?
- A 10.00 g sample containing an analyte was transferred to a 250 mL volumetric flask and diluted to volume. When a 10.00 mL aliquot of the resulting solution was diluted to 25.00 mL it was found to give a signal of 0.235 (arbitrary units). A second 10.00 mL aliquot was spiked with 10.00 mL of a 1.00 ppm standard solution of the analyte and diluted to 25.00 mL. The signal for the spiked sample was found to be 0.502. Calculate the weight percent of analyte in the original sample.Trace amounts of rare clements are found within groundwater and are of interest to goochemists. Earopium and terbium are 30 - lanthanide-scries clements that can be measured from the intensity of their fluorescence emittcd when a solution is illuminated with ultraviolet radiation. Certain organic 25 - compounds that bind Eu(III) and Tb(IIl) enhance the cmission, and substances found in natural waters can decrease 20- the cmission. For that reason it is necessary to use standard Eu (II) additions to the sample to correct for such interference. The graph shows the result of such an cxperiment in which the 15- concentration of Eu(III) and Tb(III) was mcasured in a sample of groundwatler. The x-intercept of the Eu(III) plot is 10- -23.1 ml. and the x-intercept of the Th(III) plot is -9.91 ml. In cach case, 10.00 ml. of the sample solution and 15.00 ml. 5- Tb (III) of an organic additive were added to a scries of live 50 ml. volumctric flasks. The standard Eu(lI) or Th(III) solution (0,…A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.
- Nitrite is commonly determined by a colorimetric procedure using a reaction called the Griess reaction. In this reaction, the sample containing nitrite is reacted with sulfanilimide and -(1-Napthyl)ethylenediamine to form a colored species that absorbs at 550 nm. Using an automated flow analysis instrument, the following results were obtained for standard solutions of nitrite and for a sample containing an unknown amount: Solution Absorbance at 550 nm 2.00 0.065 6.00 0.205 10.00 0.338 14.00 0.474 18.00 0.598 Unknown 0.402 (Use a spreadsheet to complete this question.) a. Find the slope, intercept, and standard deviation of the calibration curve. b. Determine the concentration of nitrite in the sample.The chromium in an aqueous sample was determined by pipetting 10.0 ml. of the unknown into each of five 50.0-mL volumetric flasks. Various volumes of a standard containing 12.2 ppm Cr were added to the flasks, following which the solutions were diluted to volume. Unknown,mLStandard, mLAbsorbancc 10.00.00.201 10.010.0 0.292 10.020.0 0.378 10.030.0 0.467 10.040.0 0.554 (a) Plot the data using a spreadsheet. (b) Determine an equation for the relationship between absorbance and volume of standard. (c) Calculate the statistics for the least-squares relationship in (b). (d) I)ctcrmine the conccnt ration oÍCr in ppm in the sample. (e) Find the standard deviation of the result in (d).A company is producing a powder which can be reconstituted as a healthy drink for the elderly. Analysis of the powder for calcium involved using the following procedure. 4 g of powder was reconstituted to 150 ml with water. Thirty ml of 24 % TCA was added to 30 ml of this solution. The mixture was allowed to stand for 15 mins and then filtered. The filtrate was analysed for calcium using atomic absorption spectroscopy. From the standard curve it was found that the reading for the analysed sample was 24mg/litre. Calculate the concentration of calcium in the powder (mg/kg).
- What is the relationship between chemical shift in ppm and operating frequency?The Beer-Lambert law you'll be using to determine the concentration of an unknown is A = ebc where A is the absorbance measured on the spectrometer, e (epsilon) is the molar absorptivity in M cm1, b is the path length of the cell (the diameter of the cuvette which is filled with solution), and c is the concentration of the solution in M. If you have a sample which has an absorbance of 1.17, a molar absorptivity of 1 Mcm1, and a cuvette that is 1.15 cm wide, what is the concentration of the sample in M? Give your answer to 3 decimal places (e.g.: 0.123)1.0 g of a compound A is prepared in 100 mL of water, and then extracted with 50 mL of ether. After the extraction, 0.25 g of the compound was recovered from the ether layer. What is the partitioning coefficient([A]ether/[A]water) for this compound in the system used? 50 ml ether 0g 0.25 g 1g 100 ml water O 4.0 O 3.0 O 1.5 O 0.75 O 0.25 O 0.67