Graph of Absorbance vs Time (mins) 1.8 1.6 1.4 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) - Group 2 (Ampicillin) Group 2(Control) Measurement of bacterial cell growth in LB Absorbance(60onm)
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- Calculate the effective quantity (g) of sodium chloride related to tonicity in 100 mL of an intravenous fluid labeled "5% dextrose in 0.45% sodium chloride," and indicate whether the solution is isotonic, hypotonic, or hypertonic.Using G-25 Sephadex beads (Fractionation range 1000-5000) KD, a sample of Vitamin B12 (MW ~1500 KD) will be: Fully excluded Partially included Fully included the activity of lysozyme was determined by looking at the increase in light scattering per unit time increase in absorbance per unit time. decrease in absorbance per unit time. increase in concentration per unit time.Rx Of oxacinophtalmic solution 3% Disp. 10 mL How many milligrams of axacin contained in each milliter of the dispensed prescription?
- Table 1 - Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract Absorbance 0 mins after 30mins (2nd reading) (mL) 1st reading 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 0.00 0.060 0.033 0-05-2 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1? Time for reading: 3:21 -0.11 Absorbance: Time for reading: 3.36 Q5) Tube la serves as a control, but why is this control needed? Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: catechol Q2) What is the substrate? Answer: THO Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: . 166 ness Catechol Benzoquinone Subtract 1st from 2nd reading -0.01 0-137 0.11.19 0.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different…What is measured at the A280 absorbance values?Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.
- Calculate the concentration of 0.2ml amino acid when path length is 1cm and has an absorbance value of 0.50, if a 0.5ml solution of same amino acid has a concentration of 0.72mg/ml with an absorbance of 0.9.When combining 1ml of lysosome solution with a concentration of 5mg/ml and 1ml of water what is the final concentration of the lysosome solution after adding 8.0 of a biuret reagent to the solution 2.5mg/ml 1mg/ml 0.5mg/ml 5 mg/mlCalculate the effective quantity (g) of sodium chloride related to tonicity in 100ml of an intravenous fluid labeled "5% dextrose in 0.45% sodium chloride," and indicate whether the solution is isotonic, hypotonic or hypertonic. The answer is Hypertonic and 1.35g NaCl but I don't understand how to get the answer.
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.K 1 1 H202 (mL) Rate (abs/secs at 500 nm) Absorbance tubes 2 &3 Absorbance tubes 4 &:Absorbance tubes 6 & Absorbance 8 & 9 Time 2. 2.5 0.001907143 0.065 0.044 0.007 0.059 0.041 3 1.75 0.001721429 20 0.017 0.027 0.008 4 1.25 0.001728571 40 0.074 0.063 0.044 0.037 5 0.75 0.001548214 60 0.117 0.103 0.085 0.079 6. 80 0.163 0.148 0.13 0.117 7. 100 0.211 0.195 0.178 0.16 8 120 Figure 1: 10 0.0025 11 12 13 etoxidaze rate 14 0.002 15 16 17 0.0015 18 19 20 21 0.001 22 23 24 25 0.0005 26 27 28 29 30 0.5 1.5 2.5 31 H202 Concentration (ml) 32 Sheet1 (wu 0os ze sas/sqe) aze