a. For the E.coli haploid genotype below, determine the phenotype for the following proteins. Assume that any genes or structural elements not shown are wildtype. lacls lacZ lacY* lacz uninducible lacY uninducible

Wild type genomic sequence of a portion of a gene and the wild type sequence of portion of the protein gene product. The protein sequence only matches partially. ТАT AAA GGG СGA ССА ССТ GсT AАT GGG АСт тTG AGG Tyr Lys Gly Arg Pro Pro Ala Pro Arg Gln Tyr Trp Note: The five amino acids at the amino end of the WT protein match the coding sequence perfectly, but not the six at the carboxyl end. This is for an obvious reason that you should figure out before starting the problem. One of the difficulties with this problem is that you do not have the DNA sequence for the carboxyl end of the protein, but that should not be a problem! And, so far, there are no mutations; nothing unusual, it is just a normal eukaryotic gene!! A mutant is found that has the following protein sequence in that part of the protein: Tyr Lys Gly Arg Thr Cys Ser Premature Stop. What is the likely mutation? between A12 and C13 insert T. between C13 and C14 insert T. A15 -> del. C13 and C14 -> del. G8 -> T. G20 -> A. T21…

Talk about the challenges involved in determining the genetic components of polygenic illnesses.   Explain complementation groups and how the biochemical underpinnings of disease are determined using them.   Hereditary illnesses of genomic instability include Werner syndrome, Bloom syndrome, XP, ataxia-telangiectasia, and Fanconi anemia. Which of these ailments has molecular mechanisms behind it? Which kind of genetic instability is connected to which disorder?.

a. How can a single eukaryotic gene give rise to several different types of mRNA molecules?b. Excluding the possible rare polycistronic message,how can a single mRNA molecule in a eukaryoticcell produce proteins with different activities?

. You need to prepare 500 μL of a 250 µg/mL bovine gamma globulin solution, for one of your protein standards in the Bradford assay. The available bovine gamma globulin in the lab is in the form of a 2 mg/mL stock solution, and it can be diluted with deionized water. Describe or show below how you would create your desired 250 µg/mL solution.

Fig 2 shows gastric organoids cultured from a TffCre; XYZI/fl mouse. Ki67 has been used to identify proliferating cells (green). Tamoxifen indicates exposure to tamoxifen to induce recombination of the TffCre. No Tamoxifen Ki-67 3. What is the consequence of deleting XYZ in gastric organoids, and does this suggest it is an oncogene or a tumour suppressor? Explain your reasoning. DX Equation Editor 4. IWP inhibits the secretion of Wnt ligands from the organoids. Therefore, do Wnt ligands promote or inhibit proliferation of XYZ deficient organoids? Explain your reasoning. X Equation Editor Tamoxifen Q Tamoxifen + IWP A- A I B IU S x, x² Styles Font Size Font Words: 0/250 张 A- A-T BIUS X, x² Styles ▾ Size

Briefly state the function of each of the lettered sites on the outlined gene below. +1 TATA Coding ААТААА А А вс E

Give typed full explanation  Ataxia telangiectasia is a neurodegenerative autosomal recessive disease that results in severe disability. It is caused by mutations in the ATM gene. The ATM protein has been shown to play a role in both NHEJ and homologous recombination. What types of DNA lesions do you expect to be present in patients with ataxia telangiectasia?   Oxidative damage   Thymine dimers   Abnormal nucleotides   Double-stranded breaks