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- 1.Why is wavelenth the main limiting factor on limit of resoltuin in light microscopy? 2.Assuming that all other variables remain constant, explain why light of shorter wavelengths will produce a clearer image that light of longer wavelenths. 3. Why aren't the magnification of both ocular lenses of a binocular microscope used to calculate total magnification?od 1 Meet- zpn-oxtp-bxu Unit 2 test - cells, organelles, mer X G In order to deteminie how cells rx A testing.illuminateed.com/assessment/5f765e7b4c2b2eb5078b7842/5f765e7b4c2b2eb5078b7843/1?rldbqn=1 ail YouTube Maps O News (1) Facebook Launch Meeting - Z... TikTok sues U.S. go... i! Spanish Present Pro... embrane, transport fall 2020 D. Water moves into and out of the cell at the same rate since the cell is isotonic to its environment. 7. In carrying out normal activities, cells use oxygen and produce carbon dioxide. The concentration of oxygen is higher in the blood than inside the cell, so oxygen moves into the cell. Similarly, carbon dioxide moves out of the cell into the blood because the concentration of carbon dioxide inside the cell is greater than the concentration outside the cell. How do the small molecules of oxygen and carbon dioxide move through the cell membrane?In less than 10 words, answer the following question. What would happen if you increased the MALDI-ToF laser pulse length from 1 ns to 1 ms?
- Phase contrast microscopy - Human Cheek Cells a. b. What is the basic principle of image formation using this microscopy technique? What can be observed and concluded from the image of the specimen?1.At which wavelength is UV radiation most germicidal? Explain. 2.What limited protection do cells have against the damaging effects of UV rediation?You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells.You are growing up myoblasts ) If 20% of a culture of human cells have a DNA content somewhere between 1&2*s (S-phase=8 hours) and 1X amount of DNA in non-dividing cells. What is the generation time?
- Models of higher-order compaction suggest that thenucleosomal fiber is _____ into a shorter but widerfiber.In your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to use a 100 fs 80 MHz laser pulse train or a 200 fs 80 MHz laser pulse train for imaging biological samples? Please explain your answer using equations 1 and 2 <I(t)2> = gP <I(t)>2 / (Rτ) ..............equa (1) τout = τin (1 + 7.68(D/τ2in)2) 1⁄2 ................equa (2) D is the total dispersion in femtoseconds squared gp is a unitless factor that depends on the temporal laser pulse shape (0.66 for a Gaussian pulse shape), τ is the full-width half-maximum (FWHM) of time average valueblackboardcdn.com/5bfc08ba3fldc/14683296?X-Blackboard-Expiration=16245468000008X-Blackboard- 19 / 47 100% 1.4. Functions of the light mlcroscope parts Complete the following table by writing the function(s) of each of the parts indicated. Structure Function Diaphragm / iris Stage opehing Lamp Objective lenses Eye piece Coarse and find adjustment knobs Stage Stage rack prt sc delete home backspace lock enter pause t shift
- What two fluorescent proteins would be least appropriate to use to visualize co-localization of two fusion proteins within a cell using fluorescence microscopy? Excitation and Emission Spectra of High-Performance Anthozoa Fluorescent Proteins 100 (a) 100 (b) 5 80 80 60 60 40 40 운 20 20 350 400 450 500 550 600 650 450 500 550 600 650 700 Excitation Wavelength (nm) Emission Wavelength (nm) TagBFP MTFP1 - Azami Green - TagBFP MTFP1 -Azami Green -TagYFP O mKO - mCherry - mPlum - TagYFP - mKO - mCherry mPlum Figure 7 O Tag BFP and mPlum Azami Green and TagYFP O TagYFP and mCherry O All of the combinations would work O TagBFP and mKO Nomalized AbsorptionQuestion 1 of 10 Question 1. If you were to engineer some non- adhesive cells to express E-cadherin at either high levels or low levels and then mixed the E-cadherin high cells with the E-cadherin low cells, what do you predict will happen?The third micrograph is at the lowest magnification. Identify A (name of the cells; blank 1). Identify B (name of the structures arrows point to; blank 2) - Identify C (name of the structures arrows point to; blank 3) High mag low mag high mag Blank # 1 Blank # 2 Blank # 3
