At what substrate concentration would an enzyme with a Km of 0.005M operate at one quarter of its Vmax? What would be the Km for an enzyme if it is operating at 90% of its Vmax when the substrate concentration is 0.01M

Q: How is the Michaelis constant defined, and what does a low or high value for Km tell you? What is…

A: Enzymes are the biocatalyst that increases the rate of the biochemical reaction by reducing the…

Q: The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Q: [S] > Km Not true for any of these conditions

A: Michaelis-Menten (MM) Kinetics relates the initial rate of a reaction with substrate concentration.…

Q: You are trying to come up with a drug to inhibit the activity of an enzyme  thought to have a role…

A: In competitive inhibition, an inhibitor molecule interferes with the binding of normal substrate to…

Q: This is the answer key from the teacher and the highlighted parts are the correct answer for the…

A: Enzymology relies on the fundamental idea of Michaelis-Menten kinetics. It covers the interactions…

Q: The enzyme BURT was found to function at V0=250 uM/min with a Km=15.0 uM when [S]=10.0 uM. What is…

A: Enzymes are protein molecules that increase the rate of reactions by decreasing the activation…

Q: Calculate the rate enhancement for the enzyme pair: Enzyme 1: Kcat (sec-1)= 5.5 KM (µ M) = 1245…

A: Enzymes are known to enhance (increase) the rate of a reaction, compared to the rate at which the…

Q: The equil ibrium constant for the attachment of a substrate to the active site of an enzyme was…

A: The equilibrium constant of a chemical reaction is the value of its reaction quotient at chemical…

Q: Most of the enzyme reactions followed the mathematical kinetic plots suggested by Michaelis-Menten…

A: The Michaelis- Menten equation is an equation used to predict the rate of product formation of a…

Q: An enzyme-catalyzed reaction is allowed to proceed for 3.4 minutes under steady-state conditions…

A: Ans- Concentration of Product = 6.3uM           Time for which the reaction has been…

Q: Án enzyme that follows Michaelis-Menten kinetics has a KM value of 3.00 µM and a kat value of 181s1.…

A: An enzyme is a type of biological catalyst that aids in the acceleration of chemical reactions.…

Q: An experiment on enzyme-catalyzed reaction was conducted in the laboratory by a student. Results…

A: The enzymes are biomolecules that are vital for a biochemical to proceed at a faster rate. The…

Q: An enzyme is present at 100 nM (nanomolar) and has a Vmax value of 25 uM/s (micromolar/second). The…

A: Michaelis–Menten kinetics is a well established model of enzyme kinetics.  The Michaelis -Menten…

Q: A hollow fiber enzyme reactor (HFER) consists of 5000 fibers. Each fiber has an internal diameter of…

A: Using hollow fibers, which are tiny, semi-permeable capillary membranes stacked in parallel array…

Q: Biochemistry Question

A: Nucleic acids are the polymer of nucleotides. Nucleotides are phosphate esters of nucleosides and…

Q: Assume that the reaction catalyzed by an enzyme follows Michaelis-Menten kinetics. The substrate…

A: Mathematical equation of the Michaelis-Menten kinetics is as follows:   V=VmaxSKm+Swhere V =velocity…

Q: Calculate KI (in units of nmol L-1) for a competitive inhibitor that has α = 1.65 when 11.0 nmol L-1…

A: α=1+​ [I]​/KIWhere:α (alpha) is the degree of inhibition[I] is the concentration of the inhibitorKI…

Q: (a) At what substrate concentration would an enzyme with a kcat of 30.0s−1 and a Km of 0.0050 M…

A: The enzyme are the biocatalysts that increase the rate of reaction by decreasing the activation…

Q: Use the data below to determine the maximum velocity [in mM/s] of a certain enzyme-catalyzed…

A: We must know the Michaelis Menten equation: V=Vmax [S]Km+ [S]

Q: Draw the Michaelis-Menten Plot and Lineweaver-Burke Plot of an enzyme in the following situation.…

A: Enzymes are biocatalysts which increase the rate of biochemical reactions. Enzymes will not…

Q: An enzyme catalyzes a reaction with a Km of 8.50 mM and a Vmax of 4.25 mM s-1. Calculate the…

A: Step 1: Step 2: Step 3: Step 4:

Q: Calculate the Km of the above enzyme.

A: This is the concept of enzyme-catalyzed reaction which is given by Michaelis-Menten. The equation…

Q: At what substrate concentration would an enzyme with a kcat of 25.0 s-1 and a KM of 3.5 mM operate…

A: This answer provides a detailed explanation of how to calculate the substrate concentration at which…

Q: An enzyme has a maximal Velocity of 110.36 uM/s.  At a substrate concentrationof 8.5 uM/s.  What is…

A:

Q: In an enzyme experiment, when the enzyme is added only to the substrate, the Vmax value is 0.828 and…

A: Enzymes are proteins with catalytic function. They are sensitive to temperature, the potential of…

Q: Calculate the slope on a Lineweaver-Burk plot (Km / Vmax) for the above enzyme (lactase). (enzyme…

A: Enzyme is a catalytic molecule that increases the rate of any chemical reaction without being…

Q: Given the following reaction and equation for the initial velocity of the reaction: k₁ k3 E+SES E +…

A: We are given two equations for initial velocity (V). V=kcat [ES]    -------(eq1)       andV=k3 [ES]…

Q: If Vmax for a reaction is 10 μM ⋅ s-1 and the KM  is 0.5 μM, what is the reaction velocity when the…

A: Enzyme reaction at different substrate concentration can be characterize by Michaelis Menten…

Q: You find in the literature that an enzyme purified very close to homogeneity has a specific activity…

A: Enzymes are catalysts that speed up biochemical reactions. Enzymes are specific to temperature,…

Q: If a data from an enzyme experiment is plotted as a Lineweaver - Burk plot, and the Vmax is 0.02…

A: Enzymes are macromolecular biological catalysts that accelerate chemical reactions. The molecules…

Q: For some Enzyme, the Vmax is 18 micromols/min, Km is 400 microM. If the substrate concentration is…

A: Velocity of the reaction defines the rate of reaction which is catalyzed by the enzyme. It depends…

Q: Given the Km and Vmax values of three enzymes, A, B and C: Enzyme Km Vmax A 2.0 x 103 M 4.0 B 4.2 x…

A: Enzymes are proteins that assist the biochemical reactions. Enzymes are sensitive to temperature,…

Q: Use the Michaelis-Menten plot to answer this question. What is the estimated value of Vmax of the…

A: Vmax : Reaction rate- when the enzyme gets fully occupied by substrate. Vmax- Maximum velocity

Q: If the enzyme lactase has a Vo of 0.40 mM per minute when [S] = 1.25 mM, and a Vo of 1.0 mM per…

A: If an enzyme follows Michaelis-Menten Kinetics, a plot of the reciprocal of the reaction velocity…

Q: How to identify the Vmax and the Michaelis constant (Km) in an enzyme kinetics graph?

A: An enzyme with a high Km requires a higher substrate concentration to achieve a given reaction…

Q: An enzyme catalyzes a reaction with a Km of 7.50 mM and a Vmax of 2.90 mM · s-!. Calculate the…

A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…

Q: If 287.9 umol of enzyme X has a Vmax = 47.8 mmol/sec, what is the value of kcat sec-1? Please report…

A: Enzymes are biological catalysts that help in catalyzing or speeding up biological reactions by…

Q: Calculate the slope on a Lineweaver-Burk plot (Km / Vmax) for the above enzyme (lactase). (enzyme…

A: Enzyme is a catalytic molecule that increases the rate of any chemical reaction without being used…

Q: You run an experiment using 0.5 µM of an enzyme. The kinetic parameters for your enzyme are kcat =…

A: Turnover number (kcat) is the maximum number of substrate molecules that are converted to product…

Q: Calculate the Km of the enzyme with these parameters.  kcat = 130s^-1 Vo = 3.0 μMs-1 S = 10 μM…

A: Km of an enzyme is known as Michalis constant, which denotes the substrate concentration at which…

Q: When 10 micrograms of an enzyme with a molecular mass of 80,000 Daultons (grams/mol) is added to a…

A: Before going into the calculations for finding the turnover number (kcat), we need to convert…

Q: For an enzyme-catalyzed reaction, the velocity was determined at two different concentrations of the…

A: The velocity of an enzyme catalyzed reaction is given by: V=Vmax×SKM+SWhere,V=velocity of the…

Q: The following data were collected in the study of a new enzyme and an inhibitor of the new enzyme:

A: The Michaelis–Menten equation is the rate equation for a one-substrate enzyme-catalyzed reaction.…

Q: you are trying to come up with a drug to inhibit the activity of an enzyme thuogth to have a role in…

A: The value for Ki′ cannot be 0/2 ×10-5, so we are assuming it as 0.2×10-5­M. We are also assuming…

Q: The Vmax for a particular enzyme is 10 nmols/L/s. The Km for its substrate is 5 microM. If the…

A: Given that, the Vmax for a particular enzyme is 10 nmol/L/s. The Km for its substrate is 5 uM. We…