Assume you have identified a new operon in bacteria (which you call the suc operon) tha encodes enzymes required for catabolism of the sugar sucrose. The structural genes that required to breakdown sucrose are transcribed from a promoter (Psuc). One of these enzymes is encoded by the sucS gene. Expression of the operon is controlled by a regulato called SucR, which binds to an operator (0). Using your knowledge of the lac operon, interpret the results of the following experiments (Table 1) to propose a model describing how the sucrose operon might be regulated. Cell genotype Production of SucS enzyme Without sucrose With sucrose R* O* p* s* ++ ot ct
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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