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Answer these questions in a You may include labeled drawings or tables, if helpful. Short answers, not long essays. 1. Is the way a protein folds random? What factors or forces determine it? 2. Explain what is meant by an enzyme's optimal temperature and explain what happens if the enzyme solution is too cold or too hot. Define what is meant by an enzyme's optimal pH and explain why extreme pH's denature most enzymes. 3. Explain the roles of the following components of the bacterial transformation/pGLO experiment: ampicillin, LB broth, heat shock, arabinose, UV light. ad animal four cheek) cells. What did each

Answer these questions in a You may include labeled drawings or tables, if helpful. Short answers, not long essays. 1. Is the way a protein folds random? What factors or forces determine it? 2. Explain what is meant by an enzyme's optimal temperature and explain what happens if the enzyme solution is too cold or too hot. Define what is meant by an enzyme's optimal pH and explain why extreme pH's denature most enzymes. 3. Explain the roles of the following components of the bacterial transformation/pGLO experiment: ampicillin, LB broth, heat shock, arabinose, UV light. ad animal four cheek) cells. What did each

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Bio 206 Lab Exam 1 Name Answer these questions in a detailed but concise manner on separate paper. You may include labeled drawings or tables, if helpful. Short answers, not long essays. 1. Is the way a protein folds random? What factors or forces determine it? 2. Explain what is meant by an enzyme's optimal temperature and explain what happens if the enzyme solution is too cold or too hot. Define what is meant by an enzyme's optimal pH and explain why extreme pH's denature most enzymes. 3. Explain the roles of the following components of the bacterial transformation/pGLO experiment: ampicillin, LB broth, heat shock, arabinose, UV light. 4. We use methylene blue and Janus B Green to stain plant cells and animal (our cheek) cells. What did each stain and which one changed color? What did the color change indicate? 5. Consider the action potential simulation (neuron). The rising portion of the graph of an action potential represents the depolarization of the neuron. Which channels are active during this portion of the graph, and what happens to the charge of the neuron at that location? The falling portion of the graph represents the repolarization of the neuron. Which channels are active during this portion of the graph, and what happens to the charge of the neuron at that location? 6. We modeled Cystic Fibrosis in lab. What is the main problem with someone with Cystic Fibrosis? What ion is involved and what happens as a result of this ion? Be specific. 7. What determines the resolution limit of a microscope? Is the purpose of the fluorescence microscope to increase the magnification beyond that of a typical light microscope? Why or why not? 8. Explain the mechanisms of the four types of fluorescence microscopy we discussed in lab: 1) GFP fusion proteins 2) immunofluorescence - direct and indirect, 3) mitotracker, and 4) phalloidin. 9. Explain the mechanisms of the three types of ELISA we discussed in lab: 1) direct, 2) indirect, 3) sandwich. Which type of ELISA did we do in lab? What is one advantage of this kind of ELISA? 10. What are negative and positive controls, and why are they so important to an ELISA test? Be sure to explain what false negative and false positive results are as part of your answer.
Transcribed Image Text:Bio 206 Lab Exam 1 Name Answer these questions in a detailed but concise manner on separate paper. You may include labeled drawings or tables, if helpful. Short answers, not long essays. 1. Is the way a protein folds random? What factors or forces determine it? 2. Explain what is meant by an enzyme's optimal temperature and explain what happens if the enzyme solution is too cold or too hot. Define what is meant by an enzyme's optimal pH and explain why extreme pH's denature most enzymes. 3. Explain the roles of the following components of the bacterial transformation/pGLO experiment: ampicillin, LB broth, heat shock, arabinose, UV light. 4. We use methylene blue and Janus B Green to stain plant cells and animal (our cheek) cells. What did each stain and which one changed color? What did the color change indicate? 5. Consider the action potential simulation (neuron). The rising portion of the graph of an action potential represents the depolarization of the neuron. Which channels are active during this portion of the graph, and what happens to the charge of the neuron at that location? The falling portion of the graph represents the repolarization of the neuron. Which channels are active during this portion of the graph, and what happens to the charge of the neuron at that location? 6. We modeled Cystic Fibrosis in lab. What is the main problem with someone with Cystic Fibrosis? What ion is involved and what happens as a result of this ion? Be specific. 7. What determines the resolution limit of a microscope? Is the purpose of the fluorescence microscope to increase the magnification beyond that of a typical light microscope? Why or why not? 8. Explain the mechanisms of the four types of fluorescence microscopy we discussed in lab: 1) GFP fusion proteins 2) immunofluorescence - direct and indirect, 3) mitotracker, and 4) phalloidin. 9. Explain the mechanisms of the three types of ELISA we discussed in lab: 1) direct, 2) indirect, 3) sandwich. Which type of ELISA did we do in lab? What is one advantage of this kind of ELISA? 10. What are negative and positive controls, and why are they so important to an ELISA test? Be sure to explain what false negative and false positive results are as part of your answer.
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