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An Fab fragment binds to lysozyme with a dissociation constant of K4 = 10 M. A 1 nM (10 M) solution of lysozyme is treated with increasing concentrations of the Fab fragment. At what concentration of added Fab will half of the lysozyme be bound to the Fab? nM

An Fab fragment binds to lysozyme with a dissociation constant of K4 = 10 M. A 1 nM (10 M) solution of lysozyme is treated with increasing concentrations of the Fab fragment. At what concentration of added Fab will half of the lysozyme be bound to the Fab? nM

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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An Fab fragment binds to lysozyme with a dissociation constant of K4 = 10-¹1 M. A 1 nM (109 M) solution of lysozyme is treated with increasing concentrations of the Fab fragment. At what concentration of added Fab will half of the lysozyme be bound to the Fab? [F] = nM
Transcribed Image Text:An Fab fragment binds to lysozyme with a dissociation constant of K4 = 10-¹1 M. A 1 nM (109 M) solution of lysozyme is treated with increasing concentrations of the Fab fragment. At what concentration of added Fab will half of the lysozyme be bound to the Fab? [F] = nM
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