All things considered, the most important factor with respect to successful PCR is. Multiple Choice primer design annealing temperature extension time quantity of template

Two techniques used to make large amounts of a segment of DNA or gene of interest are: polymerase chain reaction and DNA cloning RNA interference and DNA sequencing polymerase chain reaction and DNA sequencing polymerase chain reaction and RNA interference  RNA interference and CRISPR

Which of the following is correct regarding sequencing by synthesis? All four nucleotides are added simultaneously during the sequencing phase. All four nucleotides have fluorescent tags. Homopolymer error rates are significantly lower than other methods of second-generation sequencing. All of the above

You need to complete the following steps for an experiment. Explain whether an acrylamide gel or an agarose gel is more appropriate to use for the following experiments. Purifying tRNA from total RNA Isolating a vector insert for cloning Analyzing PCR products

The three steps involved in PCR are:   Question 4 options:   Denaturation, primer annealing, and extension   Denaturation, ligation, and extension   Denaturation, template annealing, and extension   Denaturation, primer annealing, and exhaustion