A urine culture had the following culture results: Sheep blood Columbia CNA MacConkey Swarming No growth 1. > 100,000 col/mL non-lactose fermenter 2. > 100,000 col/mL red, non-lactose fermenter What steps should be done to identify these two isolates? What do you speculate that these two isolates could be?

1- Why was the T4 and E.coli mixed together in top agar and then poured on the plate instead of just spreading the two together with a glass spreader on the plate? 2- When doing the titration and isolation of the T4 phage, E.coli diluted for you to 10e7 cells/ml. why was a diluted culture used? 3- After doing the titration of T4 phage, you find your dilution plates are all TNTC. how could this have happened? 4- Could you isolate phages that infect E.coli from raw sewerage? explain.

Prepare a dilution of phage from 1.5 x 1010 PFU/mL to 1.00 x 102 PFU/mL.  Draw a dilution scheme to achieve this serial dilution considering the following: The volumes of each dilution should be 1.0mL. It is not advisable to perform any dilutions > 1/100. The first dilution in any titre determination of stock phage should use 10µl or less of stock.

What is the microbiology laboratory test that Identifies Pseudomonas plecoglossicida and what are the results of the tests that identify it? please include your sources in MLA. examples are hemolytic tests, lipid concentrations, genomic tests etc.

Hi, I am working on qPCR quantification for specific bacteria. However, when the CT value achieve greater than 30, it always shown high CT value differences in my duplication no matter I repeat the same set of experiment for those samples with low quality. My question is, what makes this happening? The other question is, how many CT value differences is acceptable for duplication?