▪ A pharmaceutical analyst needs to do quality control of a sodium furosemide solution. The max for sodium furosemide is 271 nm. The analyst transferred 2 mL of the original solution into a 100 mL volumetric flask and made up to the mark with deionised water. 5 mL of this new solution was then pipetted into a 50 mL volumetric flask and made up to the mark with 0.1M NaOH. The absorbance of this final dilute drug solution in a cuvette with path length of 1cm is 0.250 and 41 at 271 nm = 500 (g/100ml)-¹ cm-¹. 1% ▪ Calculate the concentration of original drug solution as %w/v (g/100 mL).

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▪ A pharmaceutical analyst needs to do quality control of a
sodium furosemide solution. The max for sodium
furosemide is 271 nm. The analyst transferred 2 mL of the
original solution into a 100 mL volumetric flask and made
up to the mark with deionised water. 5 mL of this new
solution was then pipetted into a 50 mL volumetric flask
and made up to the mark with 0.1M NaOH. The
absorbance of this final dilute drug solution in a cuvette
with path length of 1cm is 0.250 and 41% at 271 nm = 500
(g/100ml)-¹ cm-¹.
▪ Calculate the concentration of original drug solution as
%w/v (g/100 mL).
Transcribed Image Text:▪ A pharmaceutical analyst needs to do quality control of a sodium furosemide solution. The max for sodium furosemide is 271 nm. The analyst transferred 2 mL of the original solution into a 100 mL volumetric flask and made up to the mark with deionised water. 5 mL of this new solution was then pipetted into a 50 mL volumetric flask and made up to the mark with 0.1M NaOH. The absorbance of this final dilute drug solution in a cuvette with path length of 1cm is 0.250 and 41% at 271 nm = 500 (g/100ml)-¹ cm-¹. ▪ Calculate the concentration of original drug solution as %w/v (g/100 mL).
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