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In te Water Level Control Experiment, what type of sensor was used and why?
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- provide at least one example where each of these micrscope ( SEM and TEM) would be the method of choice.Explain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #’s from the incubated plates. ……A serial dilution yielded TNTC in the 1:1 plate and the 1:10 plate, 10 cfu’s in the 1:100 plate and 1 cfu’s in the 1:1000 plate .Collected specimens are kept in seawater for 24h to void the gut before they are fixed for scanning electron microscopy (SEM) as below:• Fix in 1% OsO4 w/v in seawater for 1h• Transfer to distilled water, 6 changes over 12h• Store in 70% (v/v) ethanolThere are 150 specimen that need to be fixed for SEM. You'll be using Petri dishes (diam. 15cm) which need to be filled 1 cm high to cover the animals.a) How much fixative do you need to prepare in litres (to one decimal place)? Hint: need volume of cylinder - r2 π hb) How much OsO4 do you need to weigh out to make your fixative? (in g)
- Compute the cfu/g (lactic acid bacteria and coliforms) of burong mustasa samples given the following values below. Show your solution. 1st Sampling 2nd Sampling Media 10-2 10-3 10-4 10-2 10-3 10-4 TNTC. 252, 248, VRBA 28, 22, 31 26, 33 0, 0 0, 0 TNTC 232 0, 0 TNTC, TNTC TNTC, TNTC MRS 38, 42 0,0 152, 132 Volume plated: 0.1mlExplain why it is important to follow proper precautions and use aseptic technique when working with clinical samples. Why do you think that samples from urine should be tested within 2 hours of collection, or if not possible, that the sample should be refrigerated?Someone want to you an antimicrobial agent that leads 99.999% inactivation when the initial microbial load was 10^7 CFU/mL.Firstly can you determine the experimental set-up. Clarify the dilutions you made and show the viable number of CFU from each dilution.
- in the capillary electrophoresis therr are two most common methods of sample introduction explain there advantages and disadvantagesThe blood sprayed on the surface of liquid nitrogen is .A appealing method .B Thin wall method .c sand method .D CryosurgeryCalculate the concentration of the overnight stock culture in CFU/mL
- From a water sample, you must determine the number of colonies present (CFU's/ml.). However, looking at the plates of the dilutions you find some cultures with scattered colonies. Briefly explain the reason for eliminating or not considering these plates in the data collection. Short answer please..Look at the table below and answer the following questions: Culture 1/10 1/50 1/100 1/200 dilution OD Reading 1 2.0 0.36 0.18 0.11 OD Reading 2 1.9 0.35 0.18 0.08 OD Reading 3 1.8 0.33 0.16 0.09 1. Which 2 dilution levels of the culture are the best to use to compute for its optical density? 2. What is the average optical density of the culture? (use values from 2 dilution levels)Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible