A Form B Form Handedness Diameter: A Diameter: nm A Basepairs/turn Distance between bases Distance for one turn nm A A Describe the terms below A A

Horizontal sequence :VIRL Vertical sequence:MKF   Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below.  NW algorithm.    1. Complete the scoring matrix.    Scoring matrix with PAM250 scores:   V I R L M         K         F           2. Set up, initialize and complete the NW matrix.  3. Retrace, align and score alignment(s).             Use the arrows and circles for the matrix and path(s).                                                                                                                                                                                                                                                                                                                                 V I R L             M           K           F           Align and score all optimal alignments here.  PLZ  the arrows and circles for the matrix and path(s) AND SHOW ALL possible Alignment

Give 3 strand slippage and trinucleotide repeat disorder examples

Using the DNA figure below to identify structures of the following letters: A= B= C= D= E= F: Circle a nucleotide for each backbone strand A B A G G A E T

Practice Complementary Base Palring Indicate the correct base order for the complementary DNA strand by placing the correct label in each indicated base locatio Adenine Thymine Cytosine Guanine Reset

Give the DNA compliment to the following DNA strand. ATG UAC b. TAC GTG OMG

You have PGFPuv at 75ng / uL You need 1.15 ng total DNA in a final volume of 30 . Calculate how much DNA and water you will need to add to both your Enzyme Mix and your Control Mix and fill out the table below.

Good antibiotics should also inhibit human enzymes to prevent overpopulation True O False

For a DNA sample, the OD value at 260nm = 0.125, and the OD value at 280nm 0.065. Purity of DNA - OD 260/280 %3D Concentration of DNA = Abs x 50 µg/ml x dilution factor (100) 0.125 x 50 µg/ml x 100 = Edit View Insert Format Tools Table 12pt v Paragraph v BIUA 2 T'v

The concentration of digested and cleaned DNA is found to be 5μg/mL. How much of your DNA sample (in μL) would require to obtain a final concentration of 30ng.