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- 6) Pore-forming toxins are a class of protein-based toxic polymers that result in the formation of large multi-protein channels that allow the free flow of substrates and liquid in and out of a cell or organelle. Research shows that these can attach to the outer mitochondrial membrane, or on the thylakoid membranes Hollyal of chloroplasts. Meman Extracelular binding space Proteiniciati Inactive soluble T obare Lipid Activation Active P Assembly Membrane tion Pelit K Partially formed pome Nunta Inson Large molecule A Protein What would the possible consequences be if this pore-forming toxin attached to the mitochondrial outer membrane or the thylakoid membrane? Explain why. ATP Nature Reviews MicrobiologyLabel the diagram and explain the salient feature that is happening in each item. For the complexes and the mobile carriers, kindly give their alternative names Electron Transport Chain Intermembrane space Inner mitochondrial membrane Intermembrane space Mitochondrial matrix 1 2 FADH₂ NADH NAD + H Mitochondrial matrix 1. 2. 3. 4. 5. 6. 7. 6 FAD H₂O 2H* + 1/20₂ Inner mitochondrial membrane Name of the Transporter 500 2e- 13040 ALLD LC ATP ADP Essential FeaturesThe following diagrams illustrate the two aqueous spaces and the inner membrane of a mitochondrion. In which diagram is the relative concentration of protons auch that ATP synthesis could occur if ATP synthase were present? (Gold spheres represent protons.) 圈圈圈圈圈 A B D E OA OB OC OD OE intermembrane space Anner membrane matrie
- In the 1930s, some physicians prescribed low doses of a compound called dinitrophenol (DNP) to help patients lose weight. This unsafe method was abandoned after some patients died. DNP uncouples the chemiosmotic machinery by making the lipid bilayer of the inner mitochondrial membrane leaky to H+. Chemical agents that cause this effect are called uncouplers. Explain how this could cause weight loss and also death. Considering the danger, is there any use for compounds like DNP or other uncouplers?Although the outer mitochondrial membrane is permeable to all small molecules, the inner mitochondrial membrane is essentially impermeable in the absence of specific transport proteins. Consider this information answer: The ATP generated by oxidative respiration is used throughout the cell. The majority of ATP production occurs in the mitochondrial matrix. How do you think ATP is made accessible to enzymes in the cytosol and other organelles?How much energy is required to pump a mole of protons in a mitochondria with the following conditions? pH of matrix = 8.2, pH of intermembrane space 7.4, membrane potential of - 65 mV (N-side relative to P-side), temperature = 37C. Express answer to the nearest 0.1 kJ/mole.
- 12. Use Figure 4 for questions a-c below. 114 ATP synthase matrix intermembrane space Figure 4: Sketch of a mitochondrion https://upload.wikimedia.org/wikipedia/commons/1/1a/Schema mitochondrion basic.svg Bionet, CC BY-SA 4.0 , via Wikimedia Commons a. Add to the sketch to show a H* concentration gradient in an active mitochondrion. (Sketch more H* in the appropriate space. Sketch less H* in the appropriate space.) 4+ b. Sketch an arrow to represent the direction that protons flow (via diffusion) through ATP synthase. c. If the concentration of protons was equal on each side of the membrane, how would that affect the flow of protons through ATP synthase? How would that affect ATP production from glucose?In the 1930s, some physicians prescribed low doses of a compound called dinitrophenol (DNP) to help patients lose weight. This unsafe method was abandoned after some patients died. DNP uncouples the chemiosmotic machinery by making the lipid bilayer of the inner mitochondrial membrane leaky to H+ . Explain how this could cause weight loss and death.Referring to the figure below, explain why NADH yields more ATP than FADH2 does. Electron-transport and proton pump Oxidative phosphorylation Outer mitochondrial membrane H* -Intermembrane H+ H+ H+ space H* H+ H+ Cytochrome c H+ COQH, CoQ UU COQH2 CoQ JU U Inner mitochondrial membrane Ht e ATPase Complex II Complex II Complex IV Complex e ADP +P - Mitochondrial matrix NADH NAD+ FADH2 FAD АТР H+ -H+ H+ H20
- Fill in the bold using the table. Because the inner mitochondrial membrane is impermeable to L, when there is an excess of mitochondrial L, it converts to M using a TCA condensation reaction, M crosses to the cytosol using a designated transport system. In the cytosol, M reconverts to L and oxaloacetate. Oxaloacetate is subsequently converted to malate with simultaneous production of N, a prerequisite cofactor for the glycolysis pathway. This reaction is then followed by conversion of N to pyruvate which produces O, a prerequisite cofactor for the fatty acid synthesis pathway. L M N O A. ATP pyruvate malate NADPH B. ATP citrate ATP NAD+ C. acetyl CoA pyruvate NAD+ NADH D. acetyl CoA citrate NAD+ NADPH E. glucose pyruvate malate NADP+The glycerol-3-phosphate shuttle can transport cytosolic NADH equivalents into the mitochondrial matrix (see Fig. 15.11c). In this shuttle, the protons and electrons are donated to FAD, which is reduced to FADH₂. These protons and electrons are subsequently donated to coenzyme Q in the electron transport chain. End of Chapter Problem 86a How much ATP is generated per mole of glucose when the glycerol-3-phosphate shuttle is used? (Tolerance is +/- 2%) ATP are generated per glucose.Using the answer code on the right, indicate which form of energy production is being described: 1. takes place in the mitochondrial matrix 2. produces H2O as a by-product 3. results in a rich yield of ATP 4. takes place in the cytosol 5. processes acetyl-CoA 6. takes place in the mitochondrial innermembrane cristae 7. converts glucose into two pyruvate molecules 8. uses molecular oxygen 9. accomplished by the electron transport system and ATP synthase (a) glycolysis (b) citric acid cycle (c) oxidative phosphorylation